Acidic Polymer Conditioning for Neutral Capillary cIEF
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Solution Overview
Problem
Existing cIEF conditioning and regeneration techniques require significant time and can lead to performance variations between capillaries and lots, as well as fluctuations in detection times during replicate separations.
Innovation Solution
An acidic high polymer composition comprising polyethylene oxide and acetic acid is used to condition neutral capillaries, which can be absorbed quickly and provides uniform capillary coating, reducing performance variations and detection time fluctuations.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If typical conditioning and regeneration techniques are used, then capillary surface coating is achieved, but significant time is required and performance variations occur between capillaries and lots
Solution Approach 1:
The patent changes the chemical parameters of the conditioning solution by using acidic high polymer composition with specific molecular weight (at least 900,000 Daltons) and controlled acidity, which fundamentally alters the conditioning mechanism to achieve faster absorption and more consistent coating across capillaries
Solution Approach 2:
The patent employs a composite conditioning solution combining high polymer (polyethylene oxide or polyethylene glycol) with acid (acetic acid or formic acid), creating a synergistic effect that enables both rapid absorption and uniform coating, reducing inter-capillary variability
2Measurement precision
If typical conditioning techniques are used, then capillary coating is achieved, but detection time fluctuations occur between replicate separations
Solution Approach 1:
By controlling the molecular weight parameter of the high polymer (at least 900,000 Daltons) and the acid concentration, the patent achieves a conditioning solution that produces consistent coating thickness and charge distribution, leading to reproducible detection times across replicates
Solution Approach 2:
The patent creates a standardized, repeatable conditioning protocol using the acidic high polymer composition that can be consistently applied across different capillaries and batches, ensuring that each capillary receives an identical coating treatment
3Duration of action of stationary object
If conventional conditioning solutions are used, then capillary coating is achieved, but coating uniformity and lifespan are limited
Solution Approach 1:
The combination of high molecular weight polymer and acid creates a composite coating structure that is both uniform in distribution and durable in performance, with the polymer providing structural integrity and the acid providing consistent charge characteristics
Solution Approach 2:
The acidic high polymer composition performs preliminary conditioning that prepares the capillary surface for optimal coating adhesion and uniformity, ensuring long-lasting performance from the first use
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The acidic high polymer composition minimizes performance variations between capillaries and lots, reduces detection time fluctuations, and extends capillary lifespan, improving assay robustness and reproducibility.
Implementation Method 1
This acidic high polymer composition can be absorbed by a capillary faster than other cIEF conditioning solutions
Data Source
AI summary
The presently claimed and described technology is directed to an acidic high polymer composition comprising about 1.0% (w/v) polymer and about 4% (v/v) carboxylic acid. The acid high polymer composition may be used as a neutral capillary storage or conditioning solution or in a method of improving capillary isoelectric focusing (cIEF) robustness or performance. The technology is also directed to a kit comprising an acidic high polymer composition, at least one stabilizer, an anolyte, and a catholyte.