Active Protein Peptide Preparation via Enzymatic Hydrolysis and Freeze-Drying

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Solution Overview

Problem

Existing methods for preparing peptides from animal connective tissue result in denatured peptides, failing to meet the needs of the food, cosmetics, and medicinal markets for active peptides.

Innovation Solution

A method involving animal connective tissue acquisition, washing, shredding, pH adjustment, enzymolysis, filtration, nanofiltration, and vacuum ultra-low temperature freeze-drying to produce active protein peptide freeze-dried powder, ensuring high activity and purity.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If conventional extraction methods (water extraction, acid extraction, high-temperature extraction) are used to prepare peptides from connective tissue, then extraction efficiency is improved, but the peptides become denatured and lose biological activity

Engineering Contradiction:
Improveextraction efficiencyVSAvoidbiological activity
Core Design Contradiction:
ProductivityVSReliability

Solution Approach 1:

The patent changes the extraction parameters by using alkaline conditions (pH 9-11) instead of neutral or acidic conditions, and controls temperature (4-25°C) to prevent denaturation while maintaining extraction efficiency. This parameter optimization allows both high productivity and preservation of biological activity

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent replaces mechanical/thermal extraction methods with enzymatic extraction using proteases. This substitution of the extraction mechanism enables gentle breakdown of proteins into active peptides without the harsh conditions that cause denaturation, thus maintaining biological activity while achieving efficient extraction

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Reliability

If enzymatic extraction is used to maintain peptide activity, then biological activity is preserved, but extraction efficiency and productivity decrease

Engineering Contradiction:
Improvebiological activityVSAvoidextraction efficiency
Core Design Contradiction:
ReliabilityVSProductivity

Solution Approach 1:

The patent merges multiple proteases (trypsin, chymotrypsin, papain, alcalase) into a combined enzymatic system that works synergistically. This combination achieves complete protein hydrolysis faster than single enzymes, improving extraction efficiency while maintaining the gentle conditions needed for preserving biological activity

Inventive Principle:
Principle #5Merging (Combining)

3Manufacturing precision

If multiple purification steps (ultrafiltration, nanofiltration, chromatography) are implemented to increase purity, then peptide purity is improved, but process complexity and production cost increase

Engineering Contradiction:
Improvepeptide purityVSAvoidprocess complexity
Core Design Contradiction:
Manufacturing precisionVSDevice complexity

Solution Approach 1:

The patent extracts and removes unwanted components (proteins, lipids, carbohydrates, nucleic acids) through selective precipitation and filtration steps. By taking out impurities rather than progressively purifying the peptide through multiple complex steps, the method achieves high purity (90%以上) with a simpler process involving fewer unit operations

Inventive Principle:
Principle #2Taking out (Extraction)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The method efficiently prepares high-purity, active protein peptides suitable for industrial production, maintaining biological activity and functionality, suitable for various applications including medicinal and cosmetic uses.

Implementation Method 1

enzymolysis

Methodology Applied
Scientific EffectEnzymolysis: Enzyme

Implementation Method 2

vacuum ultra-low temperature freeze-drying

Methodology Applied
Scientific EffectFreeze-drying: Freeze Drying

Data Source

PatentUS12152266B2Method for preparing active protein peptide and prepared active protein peptide and use thereof
Publication Date: 2024.11.26 LIU SHUANG

AI summary

A novel method for preparing an active protein peptide from connective tissue which includes steps of: connective tissue acquisition, segmenting, washing, pulverization, pH adjustment, enzymolysis, filtration, ultrafiltration, nanofiltration concentration, sterilization, freeze-drying, etc. The connective tissue protein peptide obtained by the method of the invention has features of high peptide content, high activity, etc., and the prepared active protein peptide of the connective tissue is easily absorbed by the human body, and has functions of preventing and/or alleviating and/or treating related diseases. Further, the method includes operation steps that are simple and easy to perform, which has low energy consumption, appropriate and effective utilization of animal natural resources, as well as environmental friendly, non pollution of environment, and low production costs, which is suitable for large-scale industrial production, and the purpose of efficiently preparing an protein active peptide from connective tissue can be achieved.