Adaptive Image Processing for Cell Culture State Evaluation

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Solution Overview

Problem

Existing technologies face challenges in accurately evaluating the culture state of cells due to variations in cell structure forms at different stages of cell culture, leading to errors in image processing.

Innovation Solution

An image processing device that includes a microscope, image processing unit, and procedure selector, which performs image processing based on the state of cell structures, selecting appropriate procedures for early or terminal phases of cell culture by analyzing pixel value distributions, thereby improving accuracy.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If a single image processing procedure is used for all cell culture stages, then the device complexity is reduced and ease of operation is improved, but the measurement precision and reliability of cell culture state evaluation deteriorate due to variations in cell structure forms at different stages

Engineering Contradiction:
Improveaccuracy of cell culture state evaluationVSAvoidcomplexity of image processing system
Core Design Contradiction:
Measurement precisionVSDevice complexity

Solution Approach 1:

The image processing system dynamically adapts the processing procedure based on the detected cell culture stage. The procedure selector automatically chooses between first and second image processing procedures according to whether the culture is in an early or terminal phase, making the system flexible and adaptive rather than static

Inventive Principle:
Principle #15Dynamics

Solution Approach 2:

The system changes the image processing parameters based on the cell culture stage. By detecting variations in cell structure forms and corresponding pixel value distributions, the system adjusts the processing procedure to match the current culture stage, thereby maintaining high measurement precision across different stages

Inventive Principle:
Principle #35Parameter changes

2Measurement precision

If stage-specific image processing procedures are implemented, then the measurement precision of cell culture state evaluation is improved, but the device complexity increases due to multiple processing procedures and stage detection requirements

Engineering Contradiction:
Improveaccuracy of cell culture state evaluationVSAvoidease of using image processing system
Core Design Contradiction:
Measurement precisionVSEase of operation

Solution Approach 1:

The system performs self-service by automatically detecting the cell culture stage and selecting the appropriate image processing procedure without user intervention. The procedure selector automatically determines whether to apply the first or second processing procedure based on the detected pixel value distributions, making the system easy to operate despite its complexity

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The system uses feedback from the detected cell structure forms and pixel value distributions to automatically adjust the image processing procedure. By continuously monitoring the culture state and adapting the processing accordingly, the system maintains high precision while automating the complexity management

Inventive Principle:
Principle #23Feedback

Data Source

PatentEP3428262B1Image processing device
Publication Date: 2022.09.21 NIKON CORP
  • EP3428262B1 patent drawingFigure 1
  • EP3428262B1 patent drawingFigure 2
  • EP3428262B1 patent drawingFigure 3

AI summary

An image processing device includes an image processing unit that performs image processing on an observed image in which a cell is imaged and an image processing method selector that is configured to determine an observed image processing method for analyzing the imaged cell on the basis of information of a processed image obtained through image processing of the image processing unit.