Adaptive Scanning Path Planner for Multi-Photon Microscopy
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Solution Overview
Problem
Multi-photon excitation microscopy faces challenges in maintaining a high frame rate during wide field of view sampling, particularly in identifying and efficiently scanning regions of interest within a large field of view without compromising image quality or tissue integrity.
Innovation Solution
An automated planner and adaptive sampling system that includes a full frame scanner, segmentation module, acquisition planner, and data viewing module, utilizing a compact scanner assembly with galvanometer and resonant mirrors to optimize the scanning path and adjust in real-time, ensuring high frame rates and accurate fluorescence signal recording.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Area of stationary object
If a laser scans the entire field of view in multi-photon excitation microscopy, then the coverage area is improved, but the frame rate decreases
Solution Approach 1:
The patent divides the field of view into multiple regions of interest (ROIs) and scans only those specific regions rather than the entire field of view. This segmentation approach allows the system to maintain wide field of view coverage capability while significantly improving frame rate by concentrating scanning resources on biologically relevant areas.
2Productivity
If the scanning path is optimized for speed, then the frame rate is improved, but the scanning precision may deteriorate
Solution Approach 1:
The patent implements dynamic scanning where the scanning path and parameters are adjusted in real-time based on detected regions of interest. The system transitions from static pre-defined scanning patterns to dynamic adaptive scanning that optimizes both speed and precision by concentrating scans on moving targets while maintaining high frame rates.
Solution Approach 2:
The system uses feedback from initial scanning and biological activity detection to continuously refine and adjust the scanning path. This feedback mechanism ensures that optimized scanning paths remain accurate even as regions of interest move or change, maintaining scanning precision while preserving high frame rate performance.
3Measurement precision
If the laser power is increased to improve signal detection, then the fluorescence signal quality is improved, but tissue photodamage increases
Solution Approach 1:
The patent applies local quality by concentrating laser power only on detected regions of interest rather than distributing it across the entire field of view. This localized high-power scanning improves fluorescence signal quality from neuroblast cells while minimizing overall tissue photodamage by limiting high-intensity exposure to small, specific areas.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The system enables high frame rate imaging across a wide field of view while maintaining high resolution and reducing tissue damage, allowing for efficient identification and monitoring of regions of interest with improved image quality and real-time data presentation.
Implementation Method 1
a resonant scanner (R) driven at a resonant frequency selected from 8 KHz, 12 KHz, and 16 KHz
Implementation Method 2
a first galvanometer scanner (G1), and a second galvanometer scanner (G2)
Implementation Method 3
a compact scanner assembly in optical communication with the laser
Data Source
AI summary
The invention relates to multi-photon excitation microscopy, and in particular to a path planning module and calibration module for three mirror adaptive sampling system configured to automatically generate an optimized RGG mirror scanning path by scanning a Field of View, identifying multiple region of interest targets, and analyzing the targets to generate an optimized path. The invention also relates to methods for maintaining a high frame rate during wide field of view sampling.