Adenovirus-Specific Aptamers for Rapid, Sensitive Detection
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Solution Overview
Problem
Current methods for detecting and analyzing adenoviruses in samples such as food or water are not rapid, sensitive, or cost-effective, and existing aptamer technologies do not specifically target adenoviruses.
Innovation Solution
Development of single-stranded nucleic acid aptamers that specifically bind to at least one adenovirus type, comprising sequences SEQ ID NO:1 to 3 or variants with at least 50% sequence identity, for use in detecting, capturing, quantifying, and concentrating adenoviruses.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If molecular amplification methods (PCR) are used for adenovirus detection, then detection sensitivity is improved, but the method is not rapid and requires complex procedures
Solution Approach 1:
The invention extracts and utilizes the aptamer binding function from the complex PCR amplification process. By using aptamers that specifically bind to adenovirus capsids, the detection method directly captures and detects viral particles without requiring genetic material extraction and amplification, thereby maintaining sensitivity while dramatically reducing time and procedural complexity
Solution Approach 2:
The invention creates a simplified detection model by using aptamer-virus binding as a direct detection mechanism, copying the high specificity function of antibodies but with the advantage of nucleic acid-based aptamers that can be synthesized and stored without complex production processes
2Reliability
If immunological techniques (ELISA with antibodies) are used for adenovirus detection, then detection capability is improved, but the method is not cost-effective and binding capacity is affected by complex sample matrices
Solution Approach 1:
The invention replaces expensive, complex-to-produce monoclonal antibodies with relatively inexpensive oligonucleotide aptamers that can be synthesized through standard PCR and purification protocols. These aptamers maintain reliable binding capability while significantly reducing production costs and eliminating the need for complex antibody manufacturing processes
Solution Approach 2:
The invention changes the chemical nature of the binding agent from protein-based antibodies to nucleic acid-based aptamers. This parameter change provides resistance to complex sample matrices while maintaining specific binding capability, as aptamers have different chemical properties that make them less susceptible to interference from food and environmental sample components
3Ease of operation
If antigenic methods are used for adenovirus detection, then simplicity is maintained, but detection sensitivity is insufficient for environmental and food samples
Solution Approach 1:
The invention creates a universal detection platform using aptamers that can function in both simple and complex sample matrices. The aptamer-based method maintains operational simplicity similar to antigenic tests while achieving the sensitivity of molecular methods, working effectively across diverse sample types including environmental water, food matrices, and clinical specimens
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The aptamers provide a rapid, sensitive, and cost-effective method for adenovirus analysis, with high affinity and specificity, potentially replacing commercial monoclonal antibodies and offering long-term stability.
Implementation Method 1
a single stranded nucleic acid aptamer able to specifically bind to at least one Adenovirus type
Data Source
AI summary
A single stranded nucleic acid aptamer able to specifically bind to at least one Adenovirus type, the aptamer comprising or consisting of one of sequences SEQ ID NO:1 to 3, or variants thereof having at least 50% sequence identity. Additionally, a composition comprising the aptamer. Furthermore, use of the aptamer, for detecting, capturing, concentrating and/or quantifying at least one Adenovirus type. Still further, in vitro methods for capturing, detecting and/or quantifying at least one Adenovirus type. Further yet, a kit for detecting, quantifying, capturing and/or concentrating at least one Adenovirus type.