Adhesive Molecule SEQ ID NO 1 Mass Production via Extraction
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Solution Overview
Problem
Adhesive proteins derived from marine organisms, such as Balanomorpha and Mytilidae, face challenges in mass production and are expensive, limiting their practical application, while those from Balanomorpha have complex production processes.
Innovation Solution
A novel adhesive molecule with a specific amino acid sequence (NRVDNYKEVYNKIYNKRN) or its conservative modifications, including dihydroxyphenylalanine, is developed, which can be used to create an adhesive for bonding objects, with enhanced adherence properties.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Strength
If adhesive proteins from Mytilidae are used, then adherence performance is improved, but production cost increases and mass production becomes difficult
Solution Approach 1:
The invention extracts and utilizes only the essential adhesive domain (amino acid sequence SEQ ID NO: 1) from the complex Mytilidae adhesive proteins, separating the functional adhesive component from the rest of the protein structure. This allows production of a simplified peptide that retains adherence performance while enabling easier mass production through recombinant DNA technology in microorganisms.
Solution Approach 2:
The invention creates a simplified copy of the essential adhesive functionality by synthesizing a short peptide sequence (SEQ ID NO: 1) that replicates the adhesive properties of the complex Mytilidae proteins. This synthetic copy can be produced through genetic engineering rather than extraction from natural sources, enabling cost-effective mass production.
2Strength
If adhesive proteins from Balanomorpha are used, then adherence performance is improved, but production process complexity increases due to requiring 6 to 8 kinds of proteins
Solution Approach 1:
The invention extracts only the essential adhesive domain (SEQ ID NO: 1) from the complex Balanomorpha adhesive system, eliminating the need to produce and combine 6 to 8 different proteins. This single-peptide approach maintains adherence performance while dramatically simplifying the production process to a single recombinant expression step.
Solution Approach 2:
The invention merges the adhesive functionality of multiple Balanomorpha proteins into a single optimized peptide sequence (SEQ ID NO: 1). This consolidation integrates the essential adhesive properties that previously required multiple separate proteins, allowing production through a single genetic construct in microorganisms.
3Strength
If natural adhesive proteins are extracted and purified, then adherence performance is achieved, but production cost increases and scalability is limited
Solution Approach 1:
The invention enables the adhesive peptide to be produced through self-replication via genetic engineering. The sequence is inserted into microbial hosts (E. coli, yeast) that automatically replicate and produce the peptide through their natural metabolic processes, eliminating the need for complex extraction and purification from natural sources and enabling scalable production.
Solution Approach 2:
The invention replaces the mechanical/biological extraction and purification process with a biochemical production system based on recombinant DNA technology. Instead of extracting proteins from natural sources through labor-intensive procedures, the peptide is produced through genetic engineering and microbial fermentation, enabling automated, scalable manufacturing.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The novel adhesive molecule provides a cost-effective and practical solution for bonding objects, demonstrating strong adherence even after washing, suitable for various applications including underwater use and medical fields.
Implementation Method 1
reacting tyrosinase with the aforementioned adhesive molecule
Implementation Method 2
reacting tyrosinase with the aforementioned adhesive molecule
Data Source
AI summary
To provide novel adhesive molecules. An adhesive molecule having an amino acid sequence of SEQ ID NO. 1, or an amino acid sequence including a conservative substitution, a deletion, an insertion and/or a modification in the sequence of SEQ ID NO. 1.