Adhesive Tape Calibration Reagent Layer for Mass Spectrometry Positioning
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Solution Overview
Problem
Existing methods for atmospheric pressure ionization, such as DART and DESI, face challenges in setting a reference position for detecting chemical substances and calibrating mass spectrometers, as they require arbitrary calibration reagents and lack precise positioning capabilities.
Innovation Solution
An analysis method involving the use of an adhesive tape and a pen filled with a calibration reagent, such as polyethylene glycol or fatty acids, which can generate ions using a DART ion source apparatus, allowing for the formation of a layer on a sample for precise mass spectrometry calibration and reference positioning.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If DART or DESI methods are used for atmospheric pressure ionization, then ionization of chemical substances can be achieved, but setting a reference position for detecting chemical substance positions becomes difficult
Solution Approach 1:
The invention applies preliminary action by pre-forming a layer containing a calibration reagent on the sample before analysis. This calibration reagent layer serves as a reference position marker that can be detected during mass spectrometry, thereby solving the problem of difficult reference position setting in DART or DESI methods.
2Measurement precision
If DART or DESI methods are used for atmospheric pressure ionization, then chemical substance analysis can be performed, but mass spectrometer calibration becomes difficult
Solution Approach 1:
The invention performs calibration preparation in advance by incorporating a calibration reagent into a layer that is formed on the sample beforehand. This allows the mass spectrometer to be calibrated using the known mass spectrum of the calibration reagent during the analysis process, eliminating the need for separate calibration procedures.
Solution Approach 2:
The calibration reagent in the formed layer serves a dual purpose: it acts as both a reference standard for mass spectrometer calibration and as part of the sample analysis matrix. The system calibrates itself using the embedded calibration reagent, reducing external calibration requirements.
3Reliability
If arbitrary calibration reagents are used, then mass spectrometer calibration can be attempted, but measurement reliability decreases
Solution Approach 1:
The invention applies local quality by concentrating the calibration reagent in a specific formed layer at a defined position on the sample, rather than using arbitrary calibration reagents distributed throughout the sample. This localized placement ensures consistent detection and improves measurement reliability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate detection and calibration of chemical substances by setting a reference position for mass spectrometry, improving the precision and reliability of measurements using DART or DESI techniques.
Implementation Method 1
methods for atmospheric pressure ionization are known, methods as DART (Direct_analysis in Real Time) or DESI (Desorption Electrospray Ionization) is recently drawing attention
Implementation Method 2
DART is a method that adds protons (being generated by Penning ionization of atoms or molecules in which atoms or molecules of an electronic excited state collide with water in the atmosphere) to a sample and ionizes the sample
Implementation Method 3
forming the adhesive tape including the calibration reagent
Data Source
AI summary
There is provided an analysis method including the steps of forming a layer including a calibration reagent, that can generate ions by using a DART ion source apparatus, in a predetermined area of a sample, and performing mass spectrometry on the ions generated from an area of the sample including the layer by using DART or DESI while moving the sample having the layer formed therein.