Adipose Tissue Regenerative Cell Extraction via Dual-Stage Filtration
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Solution Overview
Problem
Current methods for extracting regenerative cellular components from adipose tissue often damage the extracellular matrix and result in the discarding of regenerative cells present in the liquid fraction, leading to inefficient recovery and potential cellular damage.
Innovation Solution
A sterile closed system method involving filtration and separation using specific cut-off values to recover regenerative cells from both the liquid and dense fractions of adipose tissue without the use of reagents, utilizing a filtering element with a first cut-off value between 40-70 microns and a separation device with a second cut-off value between 8-20 microns, and including steps of homogenization and centrifugation to extract stromal vascular fraction and extracellular matrix without damage.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If reagents (enzymes) are added to the dense fraction for digestion, then regenerative cells can be separated, but the extracellular matrix is deteriorated and cellular damage occurs
Solution Approach 1:
The patent extracts and separates the liquid fraction from the dense fraction through filtration, then processes only the liquid fraction to recover regenerative cells. This eliminates the need to add reagents to the dense fraction, thereby preventing extracellular matrix deterioration while still achieving effective cell separation and recovery
Solution Approach 2:
The patent uses a filtering element with specific cut-off values (40-70 microns for the first filter, 8-20 microns for the second filter) as an intermediary mechanism to separate cells based on size. This physical separation method replaces chemical enzymatic digestion, avoiding reagent-induced damage to the extracellular matrix while achieving pure cell separation
2Productivity
If the liquid fraction is discarded after separation from the dense fraction, then processing is simplified, but regenerative cells present in the liquid fraction are lost
Solution Approach 1:
Instead of discarding the liquid fraction, the patent implements a recovery process using two filtering elements with progressively smaller cut-off values. The first filter (40-70 microns) separates the liquid fraction from the dense fraction, and the second filter (8-20 microns) further separates regenerative cells from the liquid fraction, enabling recovery of valuable regenerative cells that would otherwise be lost
Solution Approach 2:
The patent divides the filtration process into two sequential stages using filtering elements with different cut-off values. The first stage (40-70 microns) performs initial separation, while the second stage (8-20 microns) performs fine separation to recover regenerative cells. This segmentation allows efficient processing while maximizing cell recovery
3Device complexity
If traditional filtration methods are used without specific cut-off values, then device complexity is reduced, but cell separation precision is insufficient
Solution Approach 1:
The patent specifies precise cut-off value parameters for the filtering elements (40-70 microns for the first filter, 8-20 microns for the second filter). By optimizing these physical parameters, the system achieves high cell separation precision while maintaining relatively simple device architecture, balancing complexity and performance
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method allows for the rapid and efficient recovery of regenerative cells and extracellular matrix from adipose tissue, minimizing cellular damage and processing time, while maintaining the integrity of the extracellular matrix, and is capable of processing lipoaspirate adipose tissue in a sterile closed system without reagent manipulation.
Implementation Method 1
filtering a harvested adipose tissue for recovering its liquid fraction by means of a filtering element having a first cut-off value comprised between 40 microns and 70 microns
Implementation Method 2
separating regenerative cellular components present in the liquid fraction by means of cell size segregation which blocks cells bigger than a second cut-off value ranging from 8 to 20 microns
Implementation Method 3
the digested dense fraction of the adipose tissue is subjected to centrifugation which leads to the separation of the different phases (adipose portion and fluid portion) of the dense fraction and to the precipitation of a pellet rich of regenerative cells
Data Source
AI summary
The present invention concerns a novel method and assembly for extraction of regenerative cellular components from an adipose tissue, the method comprising the steps of filtering a harvested adipose tissue for recovering its liquid fraction by means of a filtering element having a first cut-off value comprised between 40 microns and 70 microns; separating regenerative cellular components present in the liquid fraction by means of cell size segregation which blocks cells bigger than a second cut-off value ranging from 8 to 20 microns; and recovering the regenerative cells-rich fraction comprising the regenerative cellular components not having being blocked during the separating step.