Adipose Tissue Filtration for Viable Stem Cell Concentration
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Solution Overview
Problem
Current methods for extracting stem cells from adipose tissue are non-standardized, operator-dependent, and cause high cell stress, contamination, and impurity formation, leading to reduced viability and survivability of mesenchymal stem cells.
Innovation Solution
A method and device using a filtering column with oscillating radial stirrers and a vacuum dehydration-filter apparatus to process adipose tissue, ensuring aseptic conditions, eliminating impurities, and minimizing mechanical stress, allowing for selective particle size and high concentration of viable stem cells.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If manual compression forces are applied for passage and forced homogenization through multiple syringe passages, then the adipose tissue can be processed, but high cell stress is caused and stem cell viability is reduced
Solution Approach 1:
The patent replaces manual mechanical compression and forced homogenization through multiple syringe passages with a vacuum-driven filtration system. The vacuum pump creates negative pressure to pass adipose tissue through a filtration column with controlled pore sizes, eliminating the need for manual compression and multiple passages while reducing cell stress and maintaining stem cell viability.
Solution Approach 2:
The patent introduces a filtration column with controlled pore sizes as an intermediary component between the adipose tissue and the collection system. This intermediary structure allows selective passage of adipose cells while filtering out debris and reducing mechanical stress, acting as a mediator that protects stem cells during the processing procedure.
2Ease of manufacture
If multiple passages through syringe via different connectors are performed, then the adipose tissue can be homogenized, but the probability of contamination increases
Solution Approach 1:
The patent replaces multiple manual syringe passages with a single vacuum-driven filtration process. The vacuum pump maintains consistent negative pressure throughout the procedure, eliminating the need for multiple passages and reducing contamination risk while achieving effective homogenization through the filtration column's controlled pore structure.
Solution Approach 2:
The patent implements an aseptic environment with sterile filtration columns and controlled vacuum conditions, creating a protected processing space that minimizes contamination risk. The closed system with sterile barriers maintains a clean environment throughout the procedure, reducing exposure to contaminants compared to multiple open syringe passages.
3Quantity of substance
If enzymatic digestion is used to obtain high concentrations of stem cells, then stem cell concentration is improved, but processing time is delayed and implantation is postponed
Solution Approach 1:
The patent replaces enzymatic digestion with a physical vacuum filtration system. The vacuum pump creates negative pressure to pass adipose tissue through a filtration column with controlled pore sizes, rapidly separating stem cells from surrounding tissue without requiring enzymatic breakdown. This mechanical method achieves high stem cell concentration in minutes rather than requiring extended enzymatic digestion periods.
Solution Approach 2:
The patent changes the processing parameters from enzymatic action to vacuum-driven mechanical filtration. By controlling vacuum pressure levels and filtration pore sizes, the system achieves rapid separation and concentration of stem cells in a matter of minutes, dramatically reducing processing time compared to enzymatic methods while maintaining high cell concentration.
4Loss of substance
If centrifugation is used to remove liquid residues, then liquid removal is achieved, but the process is complex and time-consuming
Solution Approach 1:
The patent replaces centrifugation with vacuum-driven filtration. The vacuum pump creates negative pressure that passes through the filtration column, separating liquid residues from adipose tissue through the controlled pore structure. This method achieves effective liquid removal without requiring centrifugal force, simplifying the device requirements and reducing processing complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method achieves standardized, reproducible results with high purity and viability of mesenchymal stem cells, reducing processing time to less than 5 minutes and enhancing cell survival by minimizing mechanical stress and impurities.
Implementation Method 1
a vacuum dehydration-filter apparatus (2), provided with a filter (8), adapted to be operated by a suction pump (9)
Implementation Method 2
carrying out a separation treatment of aqueous residues R2 from said remaining wet and homogenized undersize fraction ST of the second sieve (4) by means of the filter (8) and the suction pump (9) of the vacuum dehydration-filter apparatus (2)
Implementation Method 3
subjecting said adipose tissue T1 to a first physical-mechanical treatment step by means of said plurality of sieves with progressively decreasing mesh sizes and, by means of said radial stirrer means of oscillating type
Data Source
AI summary
The invention concerns a method, and a device that can implement this method, adapted to obtain adipose based compounds, of selectively variable particle sizes, distinguished by high levels of purity and by high concentrations of viable mesenchymal stem cells, which can be used in autologous transplantation procedures mainly for regenerative purposes, as well as for filling and/or volumizing purposes.