Adipose Tissue Microfragmentation With Sterile Force Standardization
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Solution Overview
Problem
Existing methods for mechanically fragmenting adipose tissue for regenerative treatments suffer from non-standardized application of physical force, leading to variable particle size reduction, sterility issues, and lack of real-time cellular characterization, which can compromise treatment efficacy and patient safety.
Innovation Solution
A system and method for standardized mechanical processing of adipose tissue using a syringe pump and defined conduits within a Biological Safety Cabinet, ensuring sterile conditions and enabling near-real-time characterization of nucleated cells and particles, with programmable force control and enzymatic digestion for rapid cell analysis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If manual methods are used to mechanically fragment adipose tissue, then particle size reduction is achieved, but the application of physical force is non-standardized leading to variable particle size
Solution Approach 1:
The patent replaces manual mechanical force application with a syringe pump system that delivers standardized, programmable forces through defined conduits. This substitution of manual mechanical operations with a controlled mechanical system ensures consistent particle size reduction while maintaining ease of operation through automation.
Solution Approach 2:
The patent standardizes the physical force parameters by controlling flow rate, pressure, and conduit dimensions through the syringe pump system. By defining and controlling these physical parameters, the system achieves consistent particle size reduction regardless of operator variability.
2Reliability
If traditional mechanical fragmentation methods are used, then adipose tissue is processed, but sterility is compromised due to sterile breaks during connection and disconnection
Solution Approach 1:
The patent merges the lipoaspiration syringe, fragmentation conduits, and collection syringe into a integrated system that operates within a Biological Safety Cabinet. This merging eliminates the need for repeated connection and disconnection of components, thereby preventing sterile breaks while maintaining manageable device complexity through systematic integration.
Solution Approach 2:
The patent uses the Biological Safety Cabinet as an intermediary controlled environment that maintains sterility throughout the entire fragmentation process. This intermediary system allows all operations to occur within a sterile field, eliminating sterile breaks without requiring complex sterile connection procedures.
3Loss of information
If conventional processing methods are used, then adipose tissue is fragmented, but real-time cellular characterization is not available
Solution Approach 1:
The patent performs enzymatic digestion and cellular characterization in parallel with the mechanical fragmentation process rather than sequentially. By initiating digestion enzymes during fragmentation and preparing for immediate post-processing analysis, the system recovers cellular information without time loss while maintaining complete tissue processing.
Solution Approach 2:
The patent maintains continuous operation by conducting enzymatic digestion throughout the fragmentation process and immediately proceeding to post-processing analysis. This continuous workflow eliminates idle time between steps and ensures no loss of cellular information, as the tissue is constantly being processed and characterized.
4Manufacturing precision
If standardized mechanical processing with syringe pump is implemented, then particle size consistency is improved, but device complexity increases
Solution Approach 1:
The syringe pump system is designed to perform multiple functions: controlling fluid flow during fragmentation, regulating pressure, and enabling programmable force application. This multi-functionality reduces the need for separate specialized devices, thereby managing overall device complexity while achieving precise particle size control.
Solution Approach 2:
The system is divided into modular components: the syringe pump unit, defined conduits, Biological Safety Cabinet integration, and post-processing analysis modules. This segmentation allows each component to be optimized independently while maintaining overall system manageability and precision.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach eliminates sterile breaks, provides consistent particle size reduction, and allows for immediate assessment of cell viability and composition, enhancing treatment reliability and safety.
Implementation Method 1
mechanically fragment adipose tissue... application of physical force to reduce the size of adipose tissue particles
Implementation Method 2
enzymatic digestion of a portion of the microfragmented adipose tissue
Data Source
AI summary
A system and method are disclosed that allows for the standardization of the application of physical force during microfragmentation of adipose tissue with enhanced sterility. A method is also disclosed that enables an accelerated digestion of a portion of the microfragmented adipose tissue that allows for at least an estimation of the viability of the released cells, as well as a particle/cell count. A further method is disclosed to provide for assessing attributes of the microfragmented adipose tissue, including physical, biochemical and metabolic characteristics of particles/cells present in and/or isolated from the microfragmented adipose tissue.