Adipose Tissue Processing Device for Sterile ADSC Extraction
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Solution Overview
Problem
Current methods for retrieving adipose-derived stem cells (ADSCs) from adipose tissue are time-consuming, often taking several hours to several days, and are prone to contamination due to the use of enzymes and multiple processing steps, which can also damage the cells during centrifugation.
Innovation Solution
A device with a rotatable chamber featuring an annular cavity and inclined sidewalls that stratifies biologic tissue into layers based on specific gravity, allowing for gentle separation and collection of ADSCs without subjecting them to damaging forces, while also incorporating morselizing screens and filters to enhance cell extraction and purity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If centrifugation is used to separate ADSCs from fat tissue, then separation efficiency is improved, but cell viability is reduced due to damaging forces
Solution Approach 1:
The device segments the separation process into distinct functional zones: a processing chamber for initial tissue breakdown, a centrifugal separation chamber for gentle separation, and a collection system. This segmentation allows each stage to operate under optimized conditions, using lower centrifugal forces in the separation chamber to protect cell viability while maintaining overall separation efficiency.
Solution Approach 2:
The patent introduces an intermediary medium (centrifugation fluid or density gradient medium) that facilitates separation between ADSCs and fat tissue. This intermediary allows cells to be separated through density differences without direct mechanical contact or high-force centrifugation, reducing cell damage while maintaining separation efficiency.
2Manufacturing precision
If multiple processing steps are used to liberate and separate ADSCs, then separation purity is improved, but processing time increases
Solution Approach 1:
The device merges multiple processing functions into an integrated system: tissue breakdown, centrifugal separation, and cell collection occur in a single continuous operation. The processing chamber and separation chamber are connected such that liberated cells automatically progress to the separation chamber, eliminating the need for separate processing steps and reducing overall processing time while maintaining high separation purity.
Solution Approach 2:
The system maintains continuous useful action by keeping the processing and separation chambers in constant operation. Tissue is continuously processed and cells are continuously separated as they are liberated, eliminating idle time between steps. This continuous flow from processing to separation to collection significantly reduces total processing time while maintaining consistent separation purity.
3Productivity
If enzymes are used to break down adipose tissue, then cell liberation is improved, but contamination risk increases
Solution Approach 1:
The device extracts and removes enzymes from the processing system, replacing chemical breakdown methods with mechanical processing. The processing chamber uses physical means (such as mechanical shear forces or controlled compression) to liberate cells from fat tissue, eliminating the contamination risk associated with enzymatic processes while maintaining cell liberation efficiency.
Solution Approach 2:
The processing chamber and associated components are designed as single-use or disposable elements that are discarded after one use. This eliminates the need for cleaning and sterilization of enzyme-containing components, reducing contamination risk. The disposable nature also allows for optimized processing materials that may be single-use, reducing cross-contamination between samples.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device enables faster, more efficient, and sterile extraction of ADSCs with higher viability and purity, reducing contamination and processing time, and is more compact and user-friendly compared to existing methods.
Implementation Method 1
the device is configured such that upon operation at least a portion of a sample of biologic tissue present in the rotatable chamber is stratified into at least two constituent layers as a function of the differing specific gravities of the constituents of the sample of biologic tissue
Implementation Method 2
a drive unit configured to rotate the rotatable chamber about the axis and produce a centrifugal field via rotation of the rotatable chamber about the axis
Data Source
AI summary
Disclosed herein are devices and methods for processing biologic tissues, such as adipose tissue or whole blood, via centrifugation, An annular cavity is present, typically at the widest interior point of a rotatable chamber. The device is operable such that a sample of biologic tissue present in the rotatable chamber can be stratified into at least two constituent layers as a function of the differing specific gravities of the constituents and at least a portion of one of the constituent layers captured in the annular cavity.