Closed Adipose Tissue Processing for Contamination-Safe SVF Recovery
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Solution Overview
Problem
Current methods for processing adipose tissue to prepare stromal vascular fraction (SVF) and fat grafts are cumbersome and prone to errors or contamination due to the transfer of contents between different containers during various processing steps.
Innovation Solution
A multi-functional apparatus designed for both collection and processing of adipose tissue, featuring a container with separate retention and filtrate volumes separated by a filter, allowing for in-line processing without the need for intermediate transfers, and equipped with ports for suction, extraction, and mixing, facilitating efficient preparation of SVF and fat grafts.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If traditional multi-step processing methods are used to prepare SVF and fat grafts, then processing can be performed with simple equipment, but the process becomes cumbersome and prone to errors or contamination due to transfer between different containers
Solution Approach 1:
The patent combines multiple processing functions (collection, filtration, centrifugation, mixing, and extraction) into a single integrated apparatus. The container houses both the retention volume for tissue and filtrate volume for processing, eliminating the need to transfer materials between separate containers and thereby reducing contamination risk while maintaining operational reliability.
Solution Approach 2:
The apparatus is designed as a multi-functional device that can perform various processing steps including filtration through integrated filters, centrifugation using a rotor, mixing with agitation mechanisms, and extraction of SVF and fat grafts. This universal design allows one apparatus to replace multiple separate devices, reducing the complexity of managing multiple containers while improving reliability.
2Ease of operation
If multiple separate containers are used for different processing steps, then each container can be optimized for its specific function, but the transfer of contents between containers increases operational complexity and contamination risk
Solution Approach 1:
The patent merges multiple processing functions into a single container system with distinct but integrated zones for retention and filtration. This eliminates the need for manual transfer between containers, simplifying operation while simultaneously reducing contamination risk by maintaining a closed, continuous processing environment.
3Productivity
If traditional processing methods are used, then equipment requirements are simple, but processing efficiency and productivity are reduced due to multiple transfer steps
Solution Approach 1:
The integrated apparatus combines collection, filtration, centrifugation, mixing, and extraction functions in one system, eliminating multiple transfer steps and significantly improving processing efficiency. While the apparatus structure is more complex than simple containers, the integration of functions reduces overall operational complexity and enhances productivity.
Solution Approach 2:
The apparatus enables continuous processing by maintaining all processing steps within a single closed system. Tissue can be collected, filtered, centrifuged, and processed for SVF or fat graft preparation without interruption or transfer, ensuring continuous useful action and improving productivity despite the sophisticated apparatus design.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The apparatus simplifies the processing of adipose tissue by reducing contamination risks and streamlining operations, enabling efficient preparation of SVF and fat grafts with improved yield and quality.
Implementation Method 1
The internal containment volume comprises a tissue retention volume and a filtrate volume separated by a filter
Implementation Method 2
The apparatus includes a suction port that is in fluid communication with the filtrate volume and provides access to the filtrate volume for suctioning from the filtrate volume material
Data Source
AI summary
Preparation of a product comprising stromal vascular fraction cells includes washing human biological material comprising adipose in a container apparatus having an internal filter, which 5 divides an internal containment volume of the container apparatus into a tissue retention volume on one side of the filter and a filtrate volume on an opposite side of the filter, and a mixing device with at least one rotatable mixing member disposed in the tissue retention volume. The washing includes operation of the mixing device to rotate the mixing member through the human biological material within the tissue retention volume, and the washing is followed by digesting washed material within 10 the internal containment volume with added enzyme, centrifuging of the container apparatus to prepare a centrifuged pellet in the filtrate volume, selectively removing material of the pellet and preparing a product with a mixture of stromal vascular fraction cells of removed pellet material and an aqueous suspension liquid.