Adipose Tissue Progenitor Cell Isolation via Density and Chelation
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Solution Overview
Problem
Current methods for obtaining adipose tissue-derived multipotent progenitor cells (ADMPs) suffer from low collection efficiency and high contamination with erythrocytes and vascular endothelial cells, leading to decreased stem cell purity and differentiation efficiency.
Innovation Solution
A method involving the removal of erythrocytes and vascular endothelial cells from adipose tissue-derived cell populations using density methods, EDTA, and other chelators to increase the purity and yield of ADMPs, allowing for the isolation of cells expressing Islet-1 and achieving high differentiation efficiency.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional collection methods are used to obtain adipose tissue-derived somatic stem cells, then the collection process is simple and safe, but the collection efficiency is low and the purity of stem cells decreases due to contamination with erythrocytes and vascular endothelial cells
Solution Approach 1:
The cell collection process is divided into multiple sequential steps: initial collection of adipose tissue-derived cells, removal of erythrocytes through density gradient centrifugation, and removal of adherent cells through EDTA treatment. This segmentation allows each step to address specific contamination issues, progressively purifying the stem cell population while maintaining safety and simplicity
Solution Approach 2:
The invention extracts and removes specific contaminating cell types (erythrocytes and adherent cells) from the mixed cell population through targeted methods. Erythrocytes are extracted via density gradient centrifugation, and adherent cells are extracted via EDTA treatment, leaving the desired stem cells behind with high purity
2Ease of manufacture
If conventional collection methods are used, then the procedure is straightforward, but the yield of stem cells is low due to high contamination with other cell types
Solution Approach 1:
The collection procedure is segmented into straightforward sequential steps that maintain ease of execution while improving yield. Each step (density gradient centrifugation, EDTA treatment) is a simple, well-established technique that can be easily performed, yet collectively they significantly increase stem cell yield by removing contaminants
Solution Approach 2:
The invention performs preliminary removal of contaminating cells before the main stem cell culture and differentiation processes. By预先 removing erythrocytes and adherent cells, the subsequent culture yields higher proportions of viable stem cells, improving overall productivity without adding complexity to the main procedure
3Productivity
If cell populations with contaminating erythrocytes and vascular endothelial cells are cultured, then the initial collection is efficient, but the proportion of stem cells decreases over time due to higher growth efficiency of contaminating cells
Solution Approach 1:
The invention extracts contaminating cell types (erythrocytes and adherent cells) before culture begins. By removing these cells through density gradient centrifugation and EDTA treatment, the culture composition remains stable with high stem cell proportion, preventing the degradation that would occur if contaminating cells were allowed to proliferate
Solution Approach 2:
The invention applies preliminary anti-action by removing potential harmful contaminants (erythrocytes and adherent cells with higher growth efficiency) before they can negatively affect the stem cell population. This preliminary removal prevents the future problem of stem cell proportion decrease, maintaining compositional stability throughout culture
4Ease of manufacture
If cell populations with high contamination are used for differentiation induction, then the initial cell collection is straightforward, but the differentiation efficiency is remarkably low
Solution Approach 1:
The invention extracts contaminating cells before the differentiation induction step. By removing erythrocytes and adherent cells through density gradient centrifugation and EDTA treatment, the remaining cell population consists predominantly of stem cells, which respond efficiently to differentiation induction, thereby achieving high differentiation efficiency with a simple process
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method results in a cell population with a high proportion of adipose tissue-derived multipotent progenitor cells, facilitating easier culture and differentiation, and enabling the production of functional cell types such as pancreatic, hepatic, and cardiac cells.
Implementation Method 1
removing the erythrocytes and adherent cells such as vascular endothelial cells contained in the adipose tissue respectively by the density method and EDTA
Implementation Method 2
removing the erythrocytes and adherent cells such as vascular endothelial cells contained in the adipose tissue respectively by the density method and EDTA
Data Source
AI summary
Disclosed is a cell mass containing an adipose-tissue-derived multipotent progenitor cell. Also disclosed is a method for producing an adipose-tissue-derived multipotent progenitor cell from an adipose tissue, which comprises the steps of: (a) removing erythrocytes from an adipose-tissue-derived cell mass to produce a preadipose-tissue-derived multipotent progenitor cell mass; and (b) removing cells other than the adipose-tissue-derived multipotent progenitor cell from the preadipose-tissue-derived multipotent progenitor cell mass to produce the desired adipose-tissue-derived multipotent progenitor cell. Further disclosed is an adipose-tissue-derived multipotent progenitor cell produced by the method.