Adipose Tissue Splitting Kit for Stem Cell Isolation
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Solution Overview
Problem
Current methods for obtaining adipose-based stem cells are inefficient, prone to cell vitality loss, contamination, and volumetric loss, and require enzymes and laboratory settings, which increase costs and procedural time.
Innovation Solution
A kit that uses blades with different slopes and negative pressure within a sterile closed system to mechanically isolate stem cells from adipose tissue without enzymes, allowing repeated processing and maintaining the original medium, thus preventing cell exposure to air and reducing contamination risks.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If enzyme (collagenase) is used to split adipose tissue, then the tissue is effectively digested and cells are released, but the process takes longer and requires enzyme neutralization
Solution Approach 1:
The patent replaces the chemical enzyme-based system with a mechanical system consisting of a rotor-stator homogenizer. The rotor rotates at high speed within the stator, creating mechanical shear forces that fragment adipose tissue and release cells, eliminating the need for collagenase enzyme and its associated waiting and neutralization steps
Solution Approach 2:
The homogenization process uses periodic mechanical action through rotational movement of the rotor. The rotor rotates back and forth or in circular motion within the stator, applying repeated mechanical stress to the adipose tissue to achieve complete fragmentation and cell release in a short time frame
2Ease of manufacture
If adipose tissue is transferred to laboratory medium for processing, then cell isolation can be performed, but the stem cells may lose their vitality during transport and processing
Solution Approach 1:
The patent extracts the cell isolation process from the traditional laboratory medium environment and performs it directly in the patient's body using the homogenization device. The mechanical homogenization occurs in situ within the adipose tissue, eliminating the need to transfer tissue to external laboratory media that could compromise cell vitality
Solution Approach 2:
The patent introduces a sterile saline solution as an intermediary medium that maintains cell vitality during the homogenization process. This isotonic solution provides a biocompatible environment for cells while allowing mechanical processing, replacing the need for complex laboratory culture media
3Productivity
If traditional liposuction and centrifuge methods are used, then stem cells can be obtained, but volumetric loss occurs during transfer and filtration
Solution Approach 1:
The patent merges the cell fragmentation and cell collection steps into a single integrated homogenization process. The rotor-stator system both breaks down adipose tissue and suspends cells in the surrounding fluid, eliminating the need for separate transfer and centrifugation steps that cause volumetric loss
Solution Approach 2:
The patent creates a simplified version of the traditional multi-step process by using homogenization alone to achieve cell release. This single-step mechanical approach replicates the cell isolation function of the complex traditional method without requiring centrifugation, filtration, and multiple transfers
4Productivity
If enzyme is used for tissue splitting, then complete digestion is achieved, but financial burden increases due to enzyme cost and neutralization requirements
Solution Approach 1:
The patent substitutes expensive collagenase enzyme with a mechanical homogenization system. The rotor-stator apparatus uses kinetic energy to physically fragment tissue, replacing the chemical digestion process and eliminating costs associated with enzyme purchase, storage, and neutralization
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables efficient, cost-effective, and practical isolation of adipose-based stem cells with maintained vitality, reducing contamination and volumetric loss, and facilitating transplantation without enzyme use.
Implementation Method 1
by using blades with different slopes and with the help of negative pressure inside a sterile closed box
Implementation Method 2
the adipose tissues are digested (they are split) without being damaged, by the vacuum effect created by the injector applying the negative pressure
Data Source
AI summary
The present invention is a kit to be used for obtaining Adipose tissue-based stem cells, which especially is suitable for obtainment of Adipose tissue-based stem cells, which comprises a reservoir (1) through which the entrance of the adipose tissue, that is taken from a predetermined area of the individual, into the kit; characterized by comprising a first housing (2), which is located on the said reservoir (1) and enables the transmission of the adipose tissue into the kit, a second housing (3), which is located on the said reservoir (1) and which enables the second injector (40) to be positioned outside the reservoir (1) and in a perpendicular position to the reservoir (1), a third housing (4) which enables the third injector (50) to be positioned outside the reservoir (1) and in a perpendicular position to the reservoir (1), an empty body (6) which is fixed inside the reservoir (1), a first inlet (7) which enables the transmission of the adipose tissue into the body (6) by pushing of the injector, a passage (8) which can rotate its own axis and which enables the circulation of the adipose tissue that is inside the body (6) between a desired inlet inside the body (6) and another inlet which is positioned in a 90 degree angle to this inlet by this rotation movement, at least one cover (10) which enables the said movement of the passage (8) and covers the top of the reservoir (1), a second inlet (12) which comprises a first splitting element (11) that is obtained by alignment of a multiple of cutting elements (10), and a third inlet (14) which comprises at least one second splitting element (13) which enables the adipose tissue split when it enters to and exits from the injector and is obtained by alignment of a multiple of cutting elements (10).