Adipose Stem Cell Extraction Screen and Filter
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for extracting and processing adipose-derived stem cells are costly and require specialized equipment, limiting their use to large hospitals and research facilities, and making it impractical for small medical clinics to employ these technologies.
Innovation Solution
A system and method for extracting and processing adipose tissue to generate a therapeutically effective amount of adipose-derived stem cells, which includes a Mini-Stem system that allows for safe and affordable extraction of stem cells from adipose tissue, suitable for use in small medical clinics and outpatient settings.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If specialized equipment and processes are used for extracting and processing adipose-derived stem cells, then the quality and purity of stem cells is improved, but the cost and device complexity increase significantly
Solution Approach 1:
The system divides the stem cell extraction and processing into distinct modular components: a lipoaspirate collection system, a processing chamber with screen and filter, and a centrifugation system. Each module performs a specific function (collection, separation, purification), allowing the complex process to be managed through simpler, interchangeable components that maintain high stem cell purity without requiring a single complex device
Solution Approach 2:
The system introduces intermediary elements such as the screen (with 1mm pores) and filter (with 100 micron pores) as mediating structures between the lipoaspirate and the final stem cell product. These intermediaries perform the separation and purification functions that would otherwise require complex specialized equipment, enabling small clinics to achieve high purity results using standard centrifuges and simple filtration components
2Reliability
If specialized equipment and processes are used for extracting and processing adipose-derived stem cells, then the reliability of stem cell therapy is improved, but the cost increases making it inaccessible to small clinics
Solution Approach 1:
The system is designed to be universally applicable across different clinical settings by using standard, off-the-shelf components that can be found in most medical clinics. The processing chamber can handle various volumes of lipoaspirate, the screen and filter can process different tissue types, and the system integrates with standard centrifuges, making the reliable stem cell extraction method accessible to small clinics without requiring custom-built specialized equipment
Solution Approach 2:
The system employs disposable components such as the single-use processing chamber, screen, and filter that can be discarded after one use. This eliminates the need for expensive sterilization equipment and complex cleaning protocols, reducing the overall cost while maintaining reliability through consistent, pre-sterilized components that ensure high-quality stem cell extraction every time
3Quantity of substance
If adipose tissue is processed to separate pluripotent cells, then the concentration of stem cells is improved, but the processing time and loss of substance increase
Solution Approach 1:
The system performs preliminary separation by allowing the lipoaspirate to settle and form distinct layers before centrifugation. The screen is positioned to capture the fat layer, and the filter is pre-positioned to collect the cell pellet. This preliminary organization of materials before the main processing step reduces the actual processing time while achieving high stem cell concentration in the final product
4Quantity of substance
If adipose tissue is processed to separate pluripotent cells, then the concentration of stem cells is improved, but the processing time and loss of substance increase
Solution Approach 1:
The system uses a screen with 1mm pores and a filter with 100 micron pores to selectively separate components of the lipoaspirate. The porous structure allows the screen to capture the fat layer while permitting plasma and smaller particles to pass through, and allows the filter to collect the cell pellet while letting excess fluid drain away. This porous filtration approach maximizes stem cell recovery and minimizes tissue loss by precisely separating only what is necessary
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The system enables the economical and safe extraction of adipose-derived stem cells, making regenerative medicine therapies more accessible to small medical clinics and allowing for therapeutic or cosmetic applications without the need for specialized facilities.
Implementation Method 1
centrifuging the first centrifuge tube containing processed lipoaspirate to concentrate a pellet comprising an enriched stem cell fraction
Data Source
AI summary
A device that allows for either fat graft preparation or cell fraction harvest is disclosed. The device includes a first centrifuge tube configured to receive and process a biological substance, the first centrifuge tube comprising an upper cylindrical portion and a lower conical portion, a sterile tissue inlet fitting, at least one sterile processing fluid inlet fitting, a sterile suction fitting, and at least one sterile extraction port connected to a first extraction tube. The first centrifuge tube further includes an internal space including a screen being positioned therein, the screen being configured to divide the internal space in half, and a filter positioned therein, the filter being positioned below the screen in the lower conical portion of the first centrifuge tube. The device may further include a second centrifuge tube configured to receive and further process the biological substance from the first centrifuge tube. The second centrifuge tube has at least one sterile fitting, wherein the second centrifuge tube is releasably connected via the at least one sterile fitting to one of the at least one sterile extraction ports of the first centrifuge tube.