Adipose Tissue Globule Device with Automated Peristaltic Circulation
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Solution Overview
Problem
Current methods for extracting mesenchymal stromal cells from adipose tissue are unsatisfactory due to low survival rates post-cryopreservation and require operator skill, limiting their effectiveness and reproducibility.
Innovation Solution
A fully automated device for producing globules of adipose tissue, comprising a service unit and single-use kit with a working chamber, size-reducing circuit, and electronic controller, which uses mechanical means to reduce tissue size and includes a peristaltic pump system for forced circulation, ensuring sterile conditions and easy operation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If enzyme-based extraction systems are used to extract mesenchymal stromal cells from adipose tissue, then cell extraction is achieved, but the survival rate of extracted cells following cryopreservation deteriorates
Solution Approach 1:
The invention extracts and removes harmful enzymes and chemical residues from the adipose tissue sample through a filtration system, eliminating the damaging agents while preserving the viable stem cells for subsequent cryopreservation and transplantation
Solution Approach 2:
The patent introduces a sterile filtration system as an intermediary between tissue extraction and cell transplantation, using filters to separate harmful substances from viable cells, thereby improving cell survival rate without requiring complex enzymatic control
2Productivity
If manual agitation is used to activate size-reducing spheres in the working chamber, then tissue size reduction is achieved, but the yield and reproducibility deteriorate due to operator dependency
Solution Approach 1:
The device enables self-service operation where the system automatically performs tissue size reduction through peristaltic pumping and mechanical agitation, eliminating the need for operator skill in manual agitation while maintaining consistent processing yield
Solution Approach 2:
The patent replaces manual mechanical agitation with an automated peristaltic pump system that provides consistent, reproducible mechanical force for tissue size reduction, eliminating operator variability while maintaining processing effectiveness
3Extent of automation
If a single-use kit with forced circulation is used, then automation and reproducibility are improved, but device complexity increases
Solution Approach 1:
The invention segments the system into a reusable service unit and a disposable single-use kit, allowing the complex automated components to be manufactured once and reused, while the single-use portion remains simple and easy to replace, thereby managing device complexity
Solution Approach 2:
The service unit is designed as a universal platform that can process multiple single-use kits through the same peristaltic pump and filtration system, allowing the complex automated components to serve multiple purposes and reducing overall system complexity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device enhances the survival rate of stem cells by uniformly reducing adipose tissue to desired dimensions, ensuring reproducible results and easy operation, while maintaining sterile conditions and reducing operator dependency.
Implementation Method 1
a peristaltic pump system for forced circulation
Data Source
AI summary
A device for producing globules of adipose tissue, comprising a service unit and a single-use kit which may be housed in the service unit, the single-use kit comprising a working chamber, a circuit for introducing an adipose tissue sample into the working chamber, a circuit for drawing globules of adipose tissue from the working chamber, a circuit for introducing a solution for washing the tissue sample into the working chamber, and a size-reducing circuit which connects an exit route of the working chamber to an entry route of the working chamber, the service unit having an electronic controller and a means for the forced circulation of the adipose tissue sample through the size-reducing circuit.