Adjustable Filtration Element for Cell Separation from Microcarriers
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Solution Overview
Problem
Conventional methods for separating cells from microcarriers in bioreactors are costly and time-consuming, involving enzyme treatment and filtration processes that are inefficient.
Innovation Solution
An apparatus with a chamber and filtration element that allows selective extension and retraction, enabling the filtration element to move relative to the base, combined with an agitation mechanism to facilitate cell detachment and separation, using an agent to release cells from microcarriers and an inhibitor to control the process.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional separation methods (settling, enzyme treatment, filtration) are used, then cells can be separated from microcarriers, but the process is costly and time-consuming
Solution Approach 1:
The filtration element is made movable relative to the chamber base through an adjustment mechanism, allowing dynamic reconfiguration of the separation system. This enables rapid transition between filtration and non-filtration states, eliminating time-consuming manual intervention and enabling continuous operation modes that significantly reduce separation process time
Solution Approach 2:
The adjustable filtration element allows the bioreactor to operate in continuous mode where media can be exchanged without stopping the process. The filtration system remains in place and can be selectively activated, maintaining continuous cell cultivation while enabling uninterrupted media exchange and cell separation operations
2Productivity
If conventional separation methods are used, then cells can be separated from microcarriers, but the process is costly
Solution Approach 1:
The filtration element is integrated directly into the chamber structure, combining the separation function with the existing bioreactor vessel. This eliminates the need for separate external filtration systems and complex transfer procedures, reducing equipment costs and operational expenses while maintaining high separation efficiency
Solution Approach 2:
The adjustable filtration element enables the system to perform its own separation function without requiring external enzyme treatments or additional chemical agents. The mechanical adjustment and filtration process is self-contained, eliminating costs associated with enzymes, additional filtration media, and extensive processing steps
3Ease of operation
If the bioreactor is stopped for media exchange, then cell separation can be performed, but continuous operation is interrupted
Solution Approach 1:
The movable filtration element allows the system to dynamically switch between operational modes. During continuous cultivation, the filtration element can be retracted or adjusted to allow free flow. When media exchange is needed, it can be positioned to enable separation without stopping the agitation or aeration, maintaining continuous operation while facilitating media exchange
Solution Approach 2:
The adjustable filtration element serves multiple functions: it enables continuous cell separation, allows media exchange without stopping the bioreactor, and can be configured for different operating modes. This multi-functionality eliminates the need to interrupt continuous operation for routine maintenance and media exchange tasks
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method significantly reduces the time and cost of cell separation by efficiently detaching cells from microcarriers and allowing for continuous operation of bioreactors, enabling easier media exchange without stopping the process.
Implementation Method 1
a filtration element configured to filter microcarriers from the mixture
Implementation Method 2
an adjustment mechanism configured for selective extension and retraction of at least a portion of the body of the chamber to move the filtration element relative to the base of the chamber
Data Source
AI summary
An apparatus for separating cells from microcarriers comprises a chamber having a body and a base. The chamber is configured to receive a mixture comprising cells and microcarriers. The apparatus further comprises a filtration element disposed within the chamber and coupled to the body. The filtration element is configured to filter microcarriers from the mixture. The apparatus further comprises an adjustment mechanism coupled to the chamber. The adjustment mechanism is configured for selective extension and retraction of at least a portion of the body of the chamber to move the filtration element relative to the base of the chamber.