Homozygous Genotype Screening for Agouti Rabbits
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Solution Overview
Problem
Conventional breeding methods for domestic rabbits with agouti/Belgian hair color are time-consuming and laborious, often resulting in inconsistent hair color due to complex genetic interactions, and frequently produce white-colored offspring when hybridized with white rabbit breeds.
Innovation Solution
A method involving the use of specific primer sets to detect and select for homozygous genotypes of the ASIP, MC1R, and TYR genes, ensuring consistent agouti/Belgian hair color by identifying genotypes AA, EE, and CC, thereby eliminating recessive white genes and ensuring uniformity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If conventional breeding methods are used to select agouti/Belgian hair color rabbits, then hair color phenotype can be obtained, but the purification process is time-consuming and laborious
Solution Approach 1:
The invention performs genotype detection in advance before breeding occurs. By detecting the ASIP, MC1R, and TYR gene genotypes of parent rabbits beforehand, the method identifies homozygous individuals (AAEECC genotype) that will definitely produce agouti/Belgian hair color offspring. This preliminary genetic screening eliminates the need for lengthy phenotypic selection and purification processes, directly resolving the contradiction between achieving high hair color purity and reducing breeding time.
2Manufacturing precision
If conventional breeding methods are used, then rabbit population can be maintained, but hair color consistency is poor due to complex genetic interactions
Solution Approach 1:
The invention replaces the traditional mechanical/phenotypic selection system with a molecular/genetic detection system. Instead of relying on complex phenotypic observations and manual selection processes that are prone to error and inconsistency, the method uses PCR-based genotype detection to directly identify rabbits with the desired AAEECC genotype. This substitution of detection methodology achieves precise control over hair color consistency while simplifying the breeding decision-making process.
3Productivity
If agouti/Belgian hair color rabbits are hybridized with white rabbit breeds, then hybrid production is achieved, but white-colored offspring frequently appear due to recessive white genes
Solution Approach 1:
The invention introduces genotype detection as an intermediary step between parent selection and hybrid breeding. By detecting the TYR gene genotype specifically, the method identifies and selects parent rabbits that do not carry recessive white genes (cc genotype). This intermediary genetic screening ensures that only homozygous agouti/Belgian rabbits (AAEECC genotype) are used for hybrid production, guaranteeing that offspring will inherit the desired hair color phenotype and eliminating the appearance of white-colored offspring.
Data Source
AI summary
The invention provides a selecting method for domestic rabbits with a agouti/Belgian hair color based on homozygous genotype selection. The purification method is applicable to purification of various domestic rabbits with the agouti/Belgian hair color. The method includes the following steps: firstly, selecting the domestic rabbits with a hair color phenotype which is the agouti/Belgian hair color; then detecting an ASIP gene, a MC1R gene and a TYR gene at the same time by utilizing a first primer set, a second primer set and a third primer set; screening the domestic rabbits with genotypes of AA (corresponding to the ASIP gene), EE (corresponding to the MC1R gene) and CC (corresponding to the TYR gene). The domestic rabbits with the genotype of AAEECC, which are obtained by the screening method of the invention, are agouti/Belgian hair color homozygotes and the recessive white gene is eliminated.