Albumin Analysis Method Stabilizing Oxidation State
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Solution Overview
Problem
Current methods for analyzing the ratio of reduced and oxidized albumin in plasma samples are plagued by instability, poor separation, and inaccurate quantification, particularly due to the reversible nature of disulfide bonds and the need for precise temperature control, making it difficult to maintain accurate measurements over time.
Innovation Solution
A method involving pH adjustment of the sample solution to a range of 4-9, dilution within a specific range, and optional ultrafiltration or chromatography to stabilize albumin, allowing for more accurate analysis using mass spectrometry or liquid chromatography, thereby reducing oxidation and improving measurement stability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional methods are used to analyze albumin ratio, then analysis can be performed, but measurement stability deteriorates due to oxidation and reversible disulfide bond formation
Solution Approach 1:
The patent applies preliminary action by adding a reducing agent to the sample solution before analysis to prevent oxidation of albumin. This preliminary treatment ensures that the albumin remains in a reduced state throughout the analysis process, addressing the instability caused by reversible disulfide bond formation and oxidation that plagues conventional methods.
Solution Approach 2:
The patent employs parameter changes by modifying the chemical environment of the sample solution through pH adjustment and addition of reducing agents. These parameter changes create conditions that stabilize albumin in a reduced state, preventing oxidation and ensuring reliable, stable measurements throughout the analysis period.
2Object-affected harmful factors
If temperature control is strictly maintained, then oxidation can be reduced, but device complexity and operation difficulty increase
Solution Approach 1:
The patent replaces the mechanical temperature control system with a chemical approach by introducing reducing agents and adjusting pH. This substitution eliminates the need for complex temperature control equipment while achieving the same goal of preventing oxidation, thereby reducing device complexity and operational difficulty.
Solution Approach 2:
The patent changes chemical parameters (pH and reducing agent concentration) instead of relying on temperature control. This parameter change approach provides a simpler, more robust method for preventing oxidation that does not require sophisticated temperature monitoring and control systems.
3Measurement precision
If separation methods are improved, then quantification accuracy increases, but analysis time and process complexity increase
Solution Approach 1:
The patent extracts the problematic oxidation variable from the system by adding reducing agents that prevent oxidation in the first place. This extraction of the oxidation issue eliminates the need for complex separation methods to distinguish between oxidized and reduced albumin, thereby maintaining high quantification accuracy while reducing analysis time and process complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach stabilizes albumin measurements, reduces oxidation, and enables precise determination of the reduced and oxidized albumin ratio, facilitating sensitive monitoring of oxidative stress and related health conditions, even at higher temperatures, and allows for automated analysis.
Implementation Method 1
diluting the sample 50- to 100000- fold with a buffer thereby to obtain a sample solution having a pH of 4-9
Implementation Method 2
removing low-molecular compounds by a chromatography method such as affinity chromatography, gel filtration chromatography and the like, or ultrafiltration
Implementation Method 3
removing low-molecular compounds by a chromatography method such as affinity chromatography, gel filtration chromatography and the like
Implementation Method 4
subjecting the solution to mass spectrometry or liquid chromatography
Implementation Method 5
subjecting the solution to mass spectrometry or liquid chromatography
Data Source
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AI summary
The present invention provides a method of analyzing albumin in a sample solution, which is characterized by a pretreatment method of the sample solution before applying to mass spectrometry or liquid chromatography, a method of accurately and stably analyzing amount of oxidized albumin and reduced albumin in a sample solution and a presence ratio thereof, and an albumin standard sample for accuracy control of albumin quantitative analysis.