Alfalfa 410A176 Breeding via Marker-Assisted Selection

Resolve Bottlenecks,
Find Innovative Solutions
Generate Solutions

Solution Overview

Problem

Current alfalfa breeding methods face challenges in developing stable, high-yielding varieties with improved traits such as disease resistance, drought tolerance, and nutritional quality, as they often result in inbreeding depression and unpredictable genetic combinations, requiring extensive research and time to produce agronomically superior varieties.

Innovation Solution

The development of alfalfa variety 410A176, which is resistant to multiple diseases and pests, and is suitable for various cultivation conditions, involves crossing with itself or other plants to propagate and introduce transgenes for traits like herbicide tolerance, disease resistance, and improved yield, using methods like backcrossing and marker-assisted selection to maintain desirable characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional breeding methods are used to develop new alfalfa varieties, then genetic diversity and trait combination can be improved, but inbreeding depression occurs and breeding time increases

Engineering Contradiction:
Improvetrait combinationVSAvoidbreeding time
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by pre-characterizing parental lines for multiple traits (disease resistance, yield, quality) before crossing. This advance preparation allows breeders to select parents with complementary traits, reducing the time needed for subsequent selection and evaluation in the breeding program.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent implements feedback through systematic evaluation and characterization of breeding materials at multiple stages. Performance data from field trials and laboratory analyses are used to guide subsequent crossing decisions, allowing continuous improvement while avoiding inbreeding depression by identifying when genetic diversity is declining.

Inventive Principle:
Principle #23Feedback

2Reliability

If extensive crossing and selection is performed to achieve desired traits, then variety improvement is achieved, but resource consumption increases

Engineering Contradiction:
Improvevariety stabilityVSAvoidresource consumption
Core Design Contradiction:
ReliabilityVSQuantity of substance

Solution Approach 1:

The patent replaces extensive mechanical breeding operations (crossing, planting, harvesting, evaluation) with molecular marker-based selection. DNA markers associated with desirable traits allow identification of superior genotypes without requiring large-scale field trials, significantly reducing resource consumption while maintaining variety stability.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent changes the selection parameter from phenotypic evaluation (requiring growth and environmental exposure) to genotypic evaluation using molecular markers. This parameter change allows early selection in the breeding process, reducing the number of plants that need to be grown and evaluated, thereby conserving resources.

Inventive Principle:
Principle #35Parameter changes

3Object-affected harmful factors

If multiple disease resistance traits are incorporated, then disease resistance improves, but breeding complexity increases

Engineering Contradiction:
Improvedisease resistanceVSAvoidbreeding complexity
Core Design Contradiction:
Object-affected harmful factorsVSDevice complexity

Solution Approach 1:

The patent applies segmentation by breaking down the complex task of developing multi-disease-resistant varieties into separate, manageable components. Molecular markers for different disease resistance genes are identified and tracked independently, allowing breeders to accumulate multiple resistance traits through systematic selection rather than attempting to develop all traits simultaneously.

Inventive Principle:
Principle #1Segmentation

Data Source

PatentUS10531630B2Alfalfa variety 410A176
Publication Date: 2020.01.14 FORAGE GENETICS INTERNATIONAL LLC

AI summary

The invention relates to the alfalfa variety designated 410A176. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety 410A176. Also provided by the invention are tissue cultures of the alfalfa variety 410A176 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety 410A176 with itself or another alfalfa variety and plants produced by such methods.