Alfalfa Variety R411A107 Breeding via Marker-Assisted Selection

Solution Overview

Problem

Current alfalfa breeding methods face challenges in developing stable, high-yielding varieties with improved traits such as disease resistance, drought tolerance, and nutritional quality, as they often result in inbreeding depression and unpredictable genetic combinations, requiring extensive research and time to produce agronomically superior varieties.

Innovation Solution

The development of alfalfa variety R411A107, which combines traits like resistance to Aphanomyces root rot, bacterial wilt, and herbicide tolerance through the use of transgenes and specific breeding strategies, including backcrossing and marker-assisted selection, to produce seeds and plants with enhanced characteristics suitable for various agronomic practices.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional alfalfa breeding methods are used to develop new varieties, then genetic diversity is maintained, but inbreeding depression occurs and breeding cycles become extensive

Engineering Contradiction:
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent performs preliminary selection of parental plants with desired traits (disease resistance, yield, quality) before crossing. Marker-assisted selection is used early in the breeding process to identify plants carrying specific genetic markers associated with target traits, allowing breeders to make informed crossing decisions and accelerate the breeding cycle while maintaining variety stability.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent employs marker-assisted selection where DNA markers are used as feedback indicators to track the inheritance of desired traits through generations. This molecular feedback system allows breeders to monitor genetic progress and make precise selection decisions, reducing the time required to develop stable varieties compared to traditional phenotypic selection alone.

Inventive Principle:
Principle #23Feedback

2Reliability

If backcrossing and marker-assisted selection are used to introduce specific traits, then disease resistance and herbicide tolerance are improved, but genetic combination predictability increases complexity

Engineering Contradiction:
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent uses DNA markers as intermediary tools to mediate the selection process. These molecular markers serve as proxies for difficult-to-assess traits like disease resistance, allowing indirect selection through easily detectable genetic markers. This intermediary approach simplifies the overall breeding strategy by replacing complex phenotypic evaluation with straightforward molecular analysis.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent replaces traditional mechanical/phenotypic selection methods with molecular marker-based selection. Instead of visually assessing disease resistance or conducting extensive field trials, breeders use DNA marker analysis to predict trait inheritance, substituting complex phenotypic evaluation systems with simpler genotypic assessment.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

3Manufacturing precision

If extensive breeding cycles are conducted to achieve agronomic superiority, then variety traits are improved, but productivity per unit time decreases

Engineering Contradiction:
Core Design Contradiction:
Manufacturing precisionVSProductivity

Solution Approach 1:

Marker-assisted selection provides continuous feedback on genetic progress throughout the breeding process. By monitoring marker inheritance patterns across generations, breeders can track the accumulation of desired traits and make real-time adjustments to selection strategies, accelerating the rate of genetic improvement and increasing breeding productivity without sacrificing precision.

Inventive Principle:
Principle #23Feedback

Solution Approach 2:

The patent performs preliminary genetic evaluation using DNA markers before advancing plants to later breeding stages. This preliminary genotypic screening allows early identification of promising candidates, eliminating the need for extensive field testing of all plants and thereby increasing the efficiency and productivity of the breeding program while maintaining precise trait selection.

Inventive Principle:
Principle #10Preliminary action

Data Source

PatentUS10485214B2Alfalfa variety R411A107
Publication Date: 2019.11.26

AI summary

The invention relates to the alfalfa variety designated R411A107. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety R411A107. Also provided by the invention are tissue cultures of the alfalfa variety R411A107 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety R411A107 with itself or another alfalfa variety and plants produced by such methods.