Low Molecular Weight Algae Extract for Immune Cell Stimulation
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Solution Overview
Problem
Current immunopotentiation methods, such as Panagen, face issues with low bioavailability and efficiency due to large molecule size and foreign DNA recognition, leading to negative responses and loss during oral administration.
Innovation Solution
A method involving the extraction of 5-10 kD molecules from Laminaria japonica and Laminaria angustata algae, processed to enhance bioavailability, is developed, allowing for higher permeability and stability, enabling effective immune cell stimulation with reduced side effects.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If Panagen (double-stranded fragmented human genomic DNA) is used for immunopotentiation, then immune system stimulation is achieved, but bioavailability is low and losses during digestive system passage occur
Solution Approach 1:
The invention changes the molecular size parameter from large (200-6000 base pairs) to small (5-10 kD), transforming the active substance into low molecular weight fragments that can pass through the digestive system with minimal loss and achieve high bioavailability while maintaining immunopotentiation effectiveness
Solution Approach 2:
The invention segments the large genomic DNA into small molecular weight fragments (5-10 kD) through controlled degradation, creating multiple small active units that are individually capable of stimulating immune cells while avoiding the digestive system losses that affect larger molecules
2Reliability
If foreign DNA is administered, then immune stimulation occurs, but negative responses may occur due to foreign DNA recognition and interference with human cell DNA structure
Solution Approach 1:
The invention extracts only the essential immunostimulatory components from the foreign DNA structure, isolating small molecular weight fragments that can stimulate immune cells without containing the sequences that trigger foreign DNA recognition or interfere with human cell DNA structure
Solution Approach 2:
The invention uses short-lived, low molecular weight DNA fragments (5-10 kD) that can perform their immunostimulatory function quickly and then degrade harmlessly, avoiding the persistence and potential harm of larger foreign DNA molecules
3Stability of the object's composition
If large molecule size is used in immunopotentiation drugs, then structural stability is maintained, but cell penetration capability and bioavailability decrease
Solution Approach 1:
The invention optimizes the molecular size parameter to 5-10 kD, finding the optimal balance point where molecules are small enough for efficient cell penetration and high bioavailability but large enough to maintain structural stability and immunostimulatory activity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method achieves significant immune cell stimulation with enhanced bioavailability and safety, as demonstrated by increased immune cell counts and improved patient health outcomes, with a 1 ml daily oral dosage showing optimal results.
Implementation Method 1
leaching homogenate of Laminaria japonica and Laminaria angustata algae with aqueous and isobutanolic solution
Implementation Method 2
leaching homogenate of Laminaria japonica and Laminaria angustata algae with aqueous and isobutanolic solution
Implementation Method 3
placed into a vacuum evaporator and blown through with argon, then vacuum-dried
Implementation Method 4
vacuum-dried for one hour at room temperature and then at 60° C. until all liquid fraction evaporates
Implementation Method 5
ultracentrifuged at 100,000 rpm for one hour; the resulting supernatant is withdrawn and centrifuged in 10 kD filter tubes
Implementation Method 6
centrifuged in 10 kD filter tubes for one hour at 5000 rpm, after which the lower percolated fraction is withdrawn and centrifuged in 5 kD filter tubes
Data Source
AI summary
Provided herein is a substance for use in a therapeutic method for stimulating cells in the human immune system, a method for producing this substance, and a method of use of the substance for stimulating cells of the human immune system in treating various diseases involving immune system disorders.