Alkaline Pretreated Biomass pH Control for Enzymatic Hydrolysis

Resolve Bottlenecks,
Find Innovative Solutions
Generate Solutions

Solution Overview

Problem

Conventional methods for converting lignocellulosic biomass into organic acids, such as lactic acid, face challenges due to the high pH of alkaline pretreated biomass, which hinders enzymatic hydrolysis and results in inefficient production processes, including the formation of low-value salts and high water usage.

Innovation Solution

A method involving alkaline pretreatment of lignocellulosic biomass to achieve a pH between 8.0 and 14.0, followed by simultaneous saccharification and fermentation (SSF), where the alkaline pretreated biomass is used to control pH and maintain optimal conditions for microbial activity, thereby enhancing enzymatic hydrolysis and fermentation efficiency.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Ease of manufacture

If alkaline pretreatment is performed to improve biomass structure and remove lignin, then accessibility of polysaccharides for enzymatic hydrolysis is enhanced, but pH becomes too high for efficient enzymatic hydrolysis

Engineering Contradiction:
Improveaccessibility of polysaccharidesVSAvoidenzymatic hydrolysis efficiency
Core Design Contradiction:
Ease of manufactureVSEase of operation

Solution Approach 1:

The patent applies preliminary action by performing alkaline pretreatment to modify the biomass structure and remove lignin before the enzymatic hydrolysis step. This preliminary modification enhances polysaccharide accessibility, creating favorable conditions for subsequent enzymatic action while the pH adjustment bridges the gap between pretreatment and hydrolysis requirements

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent applies parameter changes by adjusting the pH from the high alkaline state (pH 8-14) achieved during pretreatment to a lower pH range suitable for enzymatic hydrolysis. This parameter transition enables the system to benefit from both the structural modifications of alkaline pretreatment and the optimal conditions for enzyme activity

Inventive Principle:
Principle #35Parameter changes

2Ease of operation

If pH is lowered by washing with water to enable enzymatic hydrolysis, then enzymatic activity is improved, but water consumption increases significantly

Engineering Contradiction:
Improveenzymatic hydrolysis efficiencyVSAvoidwater consumption
Core Design Contradiction:
Ease of operationVSLoss of substance

Solution Approach 1:

Instead of using water for pH adjustment, the patent employs acid neutralization to lower the pH from alkaline to optimal ranges for enzymatic activity. This alternative parameter change approach achieves the same operational goal (suitable pH for hydrolysis) without the substantial water consumption associated with washing methods

Inventive Principle:
Principle #35Parameter changes

3Ease of operation

If pH is lowered by neutralizing with acids to enable enzymatic hydrolysis, then enzymatic activity is improved, but low-value salts are formed as by-products

Engineering Contradiction:
Improveenzymatic hydrolysis efficiencyVSAvoidformation of low-value salts
Core Design Contradiction:
Ease of operationVSObject-generated harmful factors

Solution Approach 1:

The patent uses controlled acid neutralization to adjust pH from alkaline to optimal levels for enzymatic hydrolysis. By carefully selecting and controlling the neutralization process, the method achieves suitable pH conditions while minimizing the formation of unwanted salt by-products, balancing operational efficiency with product quality

Inventive Principle:
Principle #35Parameter changes

4Ease of manufacture

If conventional pretreatment methods are used to improve biomass structure, then polysaccharide accessibility is enhanced, but additional pH adjustment steps and water usage are required

Engineering Contradiction:
Improvepolysaccharide accessibilityVSAvoidnumber of process steps
Core Design Contradiction:
Ease of manufactureVSDevice complexity

Solution Approach 1:

The patent merges the pretreatment and pH adjustment functions into an integrated process. The alkaline pretreatment step simultaneously achieves structural modification of biomass and creates a pH state that can be efficiently adjusted to optimal levels, reducing the need for separate, standalone pH adjustment operations and minimizing overall process complexity

Inventive Principle:
Principle #5Merging (Combining)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This approach allows for efficient production of organic acids like lactic acid by maintaining a stable pH, reducing the need for additional pH adjustments and minimizing the formation of low-value salts, thus improving the overall efficiency and cost-effectiveness of the process.

Implementation Method 1

Pretreatment of lignocellulosic biomass with an alkaline agent to obtain alkaline pretreated lignocellulosic biomass with a pH of between about 8.0 and about 14.0

Methodology Applied
Scientific EffectAlkaline pretreatment:

Implementation Method 2

The main pathway to derive fermentable sugars from lignocellulose is through enzymatic hydrolysis by cellulolytic and hemicellulolytic enzymes

Methodology Applied
Scientific EffectFermentation: Fermentation

Implementation Method 3

The main pathway to derive fermentable sugars from lignocellulose is through enzymatic hydrolysis by cellulolytic and hemicellulolytic enzymes

Methodology Applied
Scientific EffectEnzymatic hydrolysis: Hydrolysis

Data Source

PatentEP2191004B1Mild alkaline pretreatment and simultaneous saccharification and fermentation of lignocellulosic biomass into organic acids
Publication Date: 2013.06.19 STICHTING DIENST LANBOUWKUNDIG ONDERZOEK
  • EP2191004B1 patent drawingFigure 1
  • EP2191004B1 patent drawingFigure 2a~2b
  • EP2191004B1 patent drawingFigure 3a~3b

AI summary

The invention relates to a method for the production of a fermentation product from lignocellulosic biomass, to a reactor to carry out the method and to use of the reactor to produce a fermentation product.