Alkaline Textile Detergent Protease for Stability and Stain Removal
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Solution Overview
Problem
Existing proteases in liquid textile washing agents with a pH of 9 to 12 suffer from insufficient catalytic activity and storage stability, making them ineffective for removing protease-sensitive stains.
Innovation Solution
A protease with specific amino acid substitutions, such as P9T, N130D, T133A, N144K, Y217M, N252T, and Q271E, and additional substitutions at positions 6, 89, 131, 166, 189, 211, or 224, enhances stability and catalytic activity in highly alkaline washing agents.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If conventional proteases are used in liquid textile washing agents with pH of 9 to 12, then the washing agent can be formulated with high alkalinity, but the protease exhibits insufficient catalytic activity and storage stability
Solution Approach 1:
The patent applies parameter changes by modifying the amino acid sequence of the protease through specific substitutions (e.g., P9T, N130D, T133A, N144K, Y217M, N252T, Q271E) to alter the enzyme's stability and catalytic activity parameters, enabling it to function effectively in highly alkaline conditions (pH 9-12) while maintaining both storage stability and catalytic performance
Solution Approach 2:
The patent creates a composite system by combining the modified protease enzyme with washing agent ingredients in a liquid formulation, where the enzyme's modified amino acid sequence provides both storage stability and catalytic activity under alkaline conditions, achieving a synergistic effect that resolves the contradiction between stability and reliability
2Reliability
If conventional proteases are used in liquid textile washing agents with pH of 9 to 12, then the washing agent can be formulated with high alkalinity, but the protease exhibits insufficient catalytic activity for removing protease-sensitive stains
Solution Approach 1:
The patent modifies the protease's amino acid sequence parameters through specific substitutions to enhance both catalytic activity and cleaning performance on protease-sensitive stains while maintaining compatibility with high alkaline pH levels, directly addressing the insufficiency of conventional proteases in this application
3Stability of the object's composition
If the protease amino acid sequence is modified to improve stability, then storage stability increases, but the complexity of the protease structure increases
Solution Approach 1:
The patent introduces specific amino acid substitutions at defined positions (P9T, N130D, T133A, N144K, Y217M, N252T, Q271E and additional positions) to improve storage stability while maintaining reasonable structural complexity, achieving enhanced stability without excessive structural complication
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The modified protease exhibits improved storage stability and increased catalytic activity, leading to enhanced cleaning performance on protease-sensitive stains in a wide temperature range.
Implementation Method 1
They act as non-specific endopeptidases and hydrolyze any acid amide bonds that are inside peptides or proteins
Implementation Method 2
at least one protease of which the amino acid sequence has been modified in particular with regard to use in textile washing agents
Data Source
AI summary
The present disclosure relates to a textile washing agent having at least one protease and a washing agent ingredient for cleaning textiles by removing protease-sensitive stains. The protease may have proteolytic activity and include an amino acid sequence having at least 70% sequence identity with the amino acid sequence given in SEQ ID NO:1 over its entire length and, in each case based on the numbering according to SEQ ID NO:1. The protease may have amino acid substitutions at the positions corresponding to positions 9, 130, 133, 144, 217, 252 and 271; and at least one additional amino acid substitution at least one of the positions corresponding to positions 6, 89, 131, 166, 189, 211 or 224. The washing agent may have a pH ranging from approximately 9 to approximately 12, measured in a 1 wt. % solution in deionized water at 20° C.