Highly Alkaline Textile Detergent With Stabilized Protease
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Solution Overview
Problem
Existing proteases are not sufficiently stable and active in highly alkaline liquid textile detergents, leading to inadequate cleaning performance on protease-sensitive stains.
Innovation Solution
A protease with specific amino acid substitutions, such as S3T, V4I, R99E, and V199I, combined with additional substitutions at positions 74, 136, 143, 154, 161, 163, 171, 200, 203, 209, 212, or 256, enhances stability and activity in detergents with a pH of 9 to 12.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional proteases are used in highly alkaline liquid textile detergents (pH 9-12), then the detergent provides alkaline cleaning capability, but the protease exhibits insufficient stability and activity leading to inadequate cleaning performance
Solution Approach 1:
The patent applies parameter changes by modifying the amino acid sequence of the protease at specific positions (3, 4, 99, 199, and combinations with positions 74, 136, 143, 154, 161, 163, 171, 200, 203, 209, 212, or 256) to optimize its performance in highly alkaline conditions. The substitutions include specific amino acid changes such as S3T, V4I, R99E, V199I combined with other position substitutions, which alter the enzyme's physical and chemical properties to enhance stability and activity at pH 9-12 while maintaining its proteolytic function for stain removal
2Productivity
If the pH of the textile detergent is increased to 9-12 for enhanced cleaning capability, then alkaline cleaning performance is improved, but protease stability and activity decrease
Solution Approach 1:
The invention resolves this contradiction by changing the biochemical parameters of the protease through targeted amino acid substitutions at positions 3, 4, 99, 199 and combinations with other positions. These modifications adjust the enzyme's isoelectric point, charge distribution, and structural stability, enabling it to maintain activity in highly alkaline pH 9-12 environments where conventional proteases would denature or lose function
3Stability of the object's composition
If protease variants with amino acid substitutions are developed to improve alkaline stability, then storage stability is enhanced, but the complexity of enzyme production and characterization increases
Solution Approach 1:
The patent manages production complexity by focusing substitutions on a limited set of critical positions (3, 4, 99, 199, and selected combinations with 74, 136, 143, 154, 161, 163, 171, 200, 203, 209, 212, or 256). This targeted approach simplifies the mutagenesis process compared to random mutagenesis, allows systematic screening of variants, and enables rational design based on the protease's three-dimensional structure and active site requirements
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The modified protease exhibits improved storage stability and catalytic activity in highly alkaline conditions, resulting in enhanced cleaning performance on protease-sensitive stains across various temperature ranges.
Implementation Method 1
They act as non-specific endopeptidases and hydrolyze any acid amide bonds that are inside peptides or proteins
Implementation Method 2
Proteases are the longest-established enzymes contained practically all modern effective detergents
Data Source
AI summary
The present disclosure relates to a textile detergent having at least one protease and a detergent ingredient for cleaning textiles by removing protease-sensitive stains. The protease may have proteolytic activity and include an amino acid sequence having at least 70% sequence identity with the amino acid sequence given in SEQ ID NO:1 over its entire length and, in each case based on the numbering according to SEQ ID NO:1. The protease may have amino acid substitutions at the positions corresponding to positions 3, 4, 99, or 199; and at least one additional amino acid substitution at at least one of the positions corresponding to positions 74, 136, 143, 154, 161, 163, 171, 200, 203, 209, 212 or 256. The textile detergent may have a pH ranging from approximately 9 to approximately 12, measured in a 1 wt. % solution in deionized water at 20° C.