Allogeneic Skin Construct for Regenerative Wound Healing

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Solution Overview

Problem

Current treatments for deep partial-thickness burns, such as autografting, are painful and carry a high risk of rejection, and existing grafts do not effectively promote healing while minimizing rejection chances.

Innovation Solution

A bioengineered allogeneic cellularized skin construct comprising allogenic cultured keratinocytes and dermal fibroblasts with murine collagen, which deposits and secretes human extracellular matrix proteins and soluble factors like growth factors and cytokines to promote regenerative wound healing.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If autografting is used to treat deep partial-thickness burns, then the patient's own skin is used to reduce rejection risk, but the patient must endure further trauma from surgical intervention

Engineering Contradiction:

Improverejection riskVSAvoidsurgical trauma

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent introduces an intermediary substance (allogeneic skin construct) that mediates between the patient's wound and the healing process. This construct serves as a temporary template that promotes regeneration without requiring the patient's own skin, thereby eliminating the need for additional surgical trauma while maintaining low rejection risk through controlled allogeneic sourcing

Inventive Principle:

Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent creates a copy of functional skin tissue using allogeneic cells and extracellular matrix. This synthetic replica performs the healing function without requiring the patient's actual skin tissue, thus avoiding the harmful effect of surgical trauma while providing reliable rejection resistance through standardized allogeneic production

Inventive Principle:

Principle #26Copying

2Reliability

If cadaver skin grafts are used, then rejection risk is reduced, but the patient must endure further trauma from surgical intervention

Engineering Contradiction:

Improverejection riskVSAvoidsurgical trauma

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent employs an intermediary allogeneic construct that bridges the gap between cadaveric grafts and autografts. This construct provides the rejection resistance of cadaveric grafts while eliminating the need for additional surgical trauma through a standardized production process that doesn't require patient skin harvesting

Inventive Principle:

Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent creates a functional copy of skin tissue using controlled allogeneic cell cultures and extracellular matrix assembly. This synthetic replica achieves the healing benefits of cadaveric grafts without requiring surgical intervention to harvest skin, thereby eliminating the harmful effect of surgical trauma

Inventive Principle:

Principle #26Copying

3Productivity

If existing grafts are used to promote healing, then wound closure is achieved, but the grafts do not effectively promote regenerative healing

Engineering Contradiction:

Improvewound closure rateVSAvoidregenerative healing promotion

Core Design Contradiction:

ProductivityVSReliability

Solution Approach 1:

The patent employs a composite structure combining allogeneic keratinocytes, dermal fibroblasts, and extracellular matrix components. This composite construct provides both immediate wound closure and promotes regenerative healing through the coordinated action of multiple cell types and matrix proteins that work together to accelerate tissue regeneration

Inventive Principle:

Principle #40Composite materials

Solution Approach 2:

The patent creates a multi-functional graft that simultaneously provides mechanical coverage for wound closure and biological signals for regenerative healing. The construct performs multiple functions: acting as a physical barrier, delivering growth factors, and stimulating tissue regeneration, thereby overcoming the limitation of single-function existing grafts

Inventive Principle:

Principle #6Universality (Multi-functionality)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The construct provides sustained secretion of healing factors, accelerates reepithelialization, and facilitates durable wound closure with a low risk of rejection, achieving up to 95% re-epithelialization in patients with deep partial-thickness burns.

Implementation Method 1

the construct deposits and/or actively produces human extracellular matrix (ECM) proteins

Methodology Applied

Scientific EffectExtracellular matrix deposition:

Implementation Method 2

the construct secretes soluble factors associated with regenerative wound healing

Methodology Applied

Scientific EffectCellular secretion:

Implementation Method 3

the construct provides sustained secretion of the soluble factors one to four hours after thawing from cryopreservation

Methodology Applied

Scientific EffectCryopreservation: Freezing

Implementation Method 4

The secreted soluble factors induce inflammation, proliferation, granulation, and remodeling of existing matrix to promote regenerative skin healing at the wound site

Methodology Applied

Scientific EffectRegenerative wound healing:

Data Source

PatentUS20240207487A1Viable bioengineered allogeneic cellularized skin constructs that secrete soluble factors associated with regenerative wound healing

Publication Date: 2024.06.27 STRATATECH CORP

AI summary

The present disclosure is directed to viable bioengineered allogeneic cellularized construct.