Alpha-Mannan Degrading Polypeptides for Biofilm Removal
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current detergents lack effective enzymes for degrading alpha-mannan, a component of biofilms and organic matter that causes staining and malodor issues in textiles and hard surfaces, leading to redeposition of soils and malodor release.
Innovation Solution
Development of polypeptides with alpha-mannan degrading activity, specifically belonging to glycosyl hydrolase families GH76, GH92, and GH99, which are highly active in degrading alpha-mannan and can be used in cleaning compositions to address these issues.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional detergent enzymes (amylases, proteases) are used, then starch and protein stains are removed effectively, but alpha-mannan containing biofilms and organic matter cannot be degraded, leading to redeposition and malodor issues
Solution Approach 1:
The patent introduces alpha-mannan degrading enzymes (alpha-mannanases from GH families 76, 92, and 99) to the detergent enzyme cocktail, enabling the formulation to degrade multiple types of stains including alpha-mannan containing biofilms, while maintaining effectiveness against traditional stains. This expands the universality of the detergent enzyme system without compromising existing functions
2Device complexity
If no alpha-mannan degrading enzyme is added, then detergent formulation remains simple, but biofilm and organic matter accumulate causing redeposition and greying of textiles
Solution Approach 1:
The patent extracts and addresses the specific problem of alpha-mannan degradation by identifying and incorporating enzymes from GH families 76, 92, and 99 that specifically target alpha-mannan substrates. This extraction of the specific enzymatic function needed resolves the harmful effects of biofilm accumulation without requiring complete reformulation of the entire detergent system
3Productivity
If alpha-mannan degrading enzymes are incorporated, then wash performance against biofilm improves, but enzyme cocktail complexity and formulation difficulty increase
Solution Approach 1:
The patent optimizes enzyme performance by identifying specific sequence motifs and structural parameters of alpha-mannanases from GH families 76, 92, and 99. By focusing on these key parameters (sequence identity, motif presence), the formulation process becomes more systematic and manageable despite the added enzyme complexity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The polypeptides effectively break down alpha-mannan, reducing redeposition of soils and malodor release, thereby improving wash performance and textile care benefits in detergents.
Implementation Method 1
The main enzyme type participating in the degradation of mannans are endo-1,4-β-mannanases (EC 3.2.1.78), which hydrolyze the internal glycoside bonds in the mannan backbone
Implementation Method 2
polypeptides having alpha-mannan degrading activity, and polynucleotides encoding the polypeptides
Data Source
AI summary
The invention relates to compositions comprising and uses of polypeptides having alpha-mannan degrading activity. The present invention relates to polypeptides having alpha-mannan degrading activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.