Alternating Composition Washing for Chromatography Trap Columns
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Solution Overview
Problem
Current methods for reducing trap column carryover in chromatography systems are either time-consuming, sacrifice sample throughput, or are environmentally unfriendly, such as using disposable trap columns.
Innovation Solution
A method involving a washing procedure that uses alternating washing liquids with different compositions, where the first composition consists of at least 70% of a solvent and the second composition contains minimal solvent, applied in a series of pick-up cycles to effectively remove carryover while maintaining sample throughput.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If disposable single use trap columns are used, then trap column carryover is eliminated, but effort and environmental friendliness deteriorate
Solution Approach 1:
The patent changes the chemical parameters of washing liquids by using alternating compositions (e.g., organic solvent vs. aqueous buffer) to effectively remove carryover from reusable trap columns, eliminating the need for disposable columns while maintaining reliability
2Reliability
If a blank run is performed following the sample, then trap column carryover is reduced, but sample throughput deteriorates
Solution Approach 1:
The patent performs preliminary washing actions during the sample run itself by diverting portions of mobile phase through the trap column, thereby reducing carryover before the next sample is injected without requiring additional blank runs between samples
3Reliability
If manual programming of washing procedure is performed, then trap column carryover is reduced, but time consumption deteriorates
Solution Approach 1:
The patent implements self-service by enabling the system to automatically select and execute appropriate washing procedures based on the detected sample type, eliminating the need for manual programming while maintaining effective carryover removal
4Reliability
If alternating washing liquids with different compositions are used, then trap column carryover is reduced and sample throughput is maintained, but system complexity increases
Solution Approach 1:
The patent applies multi-functionality by using the existing mobile phase components (organic solvent and aqueous buffer) already present in the chromatography system for washing purposes, thereby implementing complex alternating washing procedures without adding dedicated washing equipment or increasing overall system complexity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach reduces trap column carryover significantly, enhancing data confidence and sample throughput without the need for frequent disposable columns, and is environmentally friendly.
Implementation Method 1
solid phase extraction (SPE) trap columns
Implementation Method 2
providing a first washing liquid with a first composition towards the element, and providing a second washing liquid with a second composition towards the element
Data Source
AI summary
A method of washing an element in a chromatography system, wherein the method includes performing an element rinse step. The element rinse step includes providing a first washing liquid with a first composition towards the element, and providing a second washing liquid with a second composition towards the element, wherein the second composition is different from the first composition.