Aluminum Phosphate CpG Adjuvant Composition for Antigen Adsorption

Resolve Bottlenecks,
Find Innovative SolutionsGenerate Solutions

Solution Overview

Problem

Current aluminum phosphate adjuvants have poor adsorption capacity for CpG oligodeoxynucleotides and antigens, and existing desorption methods can degrade antigens, complicating vaccine quality verification.

Innovation Solution

A novel adjuvant system is developed using aluminum phosphate adjuvants with an isoelectric point of 7.0 to 9.0, combined with CpG, enhancing adsorption and desorption efficiency, and an optimized desorption method using specific solutions to maintain antigen integrity.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Quantity of substance

If aluminum phosphate adjuvants with conventional isoelectric points (4-5) are used, then the adjuvant system is simple and cost-effective, but the adsorption capacity for CpG and antigens is poor

Engineering Contradiction:

Improveadsorption capacity for CpG and antigensVSAvoidadjuvant formulation complexity

Core Design Contradiction:

Quantity of substanceVSDevice complexity

Solution Approach 1:

The patent changes the isoelectric point parameter of aluminum phosphate adjuvant from conventional 4-5 to 7.0-9.0 through controlled precipitation conditions (pH 7.0-9.0, specific Al3+:PO43- ratios). This parameter change transforms the surface charge from negative to positive, enabling effective adsorption of negatively charged CpG and antigens while maintaining formulation simplicity

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The patent creates a composite adjuvant system combining aluminum phosphate with CpG oligodeoxynucleotides. The aluminum phosphate component provides structural support and antigen adsorption, while CpG provides immunostimulatory activity. The composite achieves synergistic effects with enhanced adsorption capacity and immunogenicity

Inventive Principle:

Principle #40Composite materials

2Quantity of substance

If existing desorption methods using strong acids or bases are used, then the adsorbed antigens can be released, but the antigens are degraded and quality verification becomes complicated

Engineering Contradiction:

Improvedesorption efficiency of antigensVSAvoidantigen integrity for quality verification

Core Design Contradiction:

Quantity of substanceVSReliability

Solution Approach 1:

The patent changes the desorption condition parameters from extreme pH (strong acids/bases) to mild pH (6.5-7.5). This parameter change allows effective desorption of antigens from the aluminum phosphate surface while maintaining antigen structural integrity and biological activity, enabling reliable quality verification

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The patent uses phosphate buffer as an intermediary desorption medium. The buffer provides a controlled chemical environment that facilitates antigen release from the adjuvant surface through competitive binding or charge neutralization, while the mild conditions preserve antigen integrity unlike direct acid/base treatment

Inventive Principle:

Principle #24Intermediary (Mediator)

3Quantity of substance

If aluminum hydroxide adjuvants are used with CpG, then the adsorption capacity is sufficient, but the formulation is less effective for certain antigens compared to optimized aluminum phosphate

Engineering Contradiction:

Improveadsorption capacityVSAvoidcompatibility with different vaccine antigens

Core Design Contradiction:

Quantity of substanceVSAdaptability or versatility

Solution Approach 1:

The patent optimizes multiple parameters of aluminum phosphate including isoelectric point (7.0-9.0), particle size (controlled precipitation), and composition (Al3+:PO43- ratio). These parameter optimizations enhance adsorption capacity and provide better adaptability to different antigen types compared to aluminum hydroxide, while maintaining CpG compatibility

Inventive Principle:

Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The system achieves high adsorption and efficient desorption of antigens and CpG, facilitating vaccine preparation and quality evaluation without interference, offering improved immunogenicity and safety.

Implementation Method 1

The aluminum phosphate adjuvant, presenting in the form of positively charged micron particles in neutral pH solutions (pH 6-7.4), and can sufficiently adsorb negatively charged CpG and negatively charged antigens

Methodology Applied

Scientific EffectElectrostatic interaction: Ion Repulsion/Attraction