Ammonia Oxidizing Bacteria Evaluation for Pathogen Suppression
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Solution Overview
Problem
There is a need for improved beneficial bacteria that can effectively suppress the growth of non-autotrophic pathogenic bacteria, as existing methods are inadequate in this regard.
Innovation Solution
The method involves evaluating the level of ammonia oxidizing bacteria in cosmetic or therapeutic products by measuring their concentration and comparing it to a predetermined range, with classifications as accepted or not accepted based on this evaluation, using specific ammonia oxidizing bacteria strains like Nitrosomonas eutropha, and employing activation and actuation processes to enhance their growth and storage viability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If existing beneficial bacteria are used to suppress pathogenic bacteria, then disease prevention is achieved, but the effectiveness against non-autotrophic pathogenic bacteria is inadequate
Solution Approach 1:
The patent changes the fundamental parameter of bacterial metabolism from heterotrophic to autotrophic by selecting ammonia oxidizing bacteria that perform chemosynthesis. This metabolic parameter change enables the bacteria to suppress non-autotrophic pathogenic bacteria through competition for ammonia and production of toxic metabolites like nitric oxide, thereby resolving the inadequacy of existing beneficial bacteria against non-autotrophic pathogens
2Reliability
If ammonia oxidizing bacteria are used to suppress non-autotrophic pathogenic bacteria, then suppression effectiveness is improved, but the viability and activity of ammonia oxidizing bacteria in cosmetic and therapeutic products is challenging to maintain
Solution Approach 1:
The patent applies preliminary action by pre-adapting ammonia oxidizing bacteria to cosmetic and therapeutic formulations before product use. The bacteria are acclimatized to the specific product environment (pH, nutrients, temperature) during manufacturing, ensuring their viability and activity are maintained when the product is applied. This preliminary adaptation resolves the stability challenge of maintaining bacterial activity in finished cosmetic and therapeutic products
3Ease of manufacture
If conventional bacteria are used in cosmetic and therapeutic products, then product formulation is straightforward, but the ability to effectively suppress non-autotrophic pathogenic bacteria is insufficient
Solution Approach 1:
The patent uses ammonia as an intermediary substance that enables both the beneficial function and maintains formulation simplicity. Ammonia serves as the energy source for autotrophic bacteria, allowing them to outcompete non-autotrophic pathogens. The intermediary ammonia connection allows the bacteria to thrive in cosmetic formulations without requiring complex nutrient formulations, thus maintaining ease of manufacture while achieving reliable pathogenic bacteria suppression
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach ensures the presence of effective ammonia oxidizing bacteria in products, which can safely and effectively suppress pathogenic bacteria by maintaining their viability and activity, thereby enhancing product quality and safety.
Implementation Method 1
The conversion of ammonia to nitrite takes place in two steps catalyzed respectively by ammonia monooxygenase (Amo) and hydroxylamine oxidoreductase (Hao)
Implementation Method 2
ammonia oxidizing bacteria with a unique capacity to generate energy exclusively from the conversion of ammonia to nitrite
Data Source
AI summary
This disclosure provides, inter alia, systems and methods for testing products for ammonia oxidizing bacteria, quality control, and providing products with ammonia oxidizing bacteria. The systems and methods herein may be used, inter alia, to treat diseases associated with low nitrite levels, skin diseases, and diseases caused by pathogenic bacteria.