Nanopore Biomolecule Analysis With Ammonium-Sulfate Electrolytes
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Solution Overview
Problem
Existing nanopore DNA sequencing methods suffer from noise interference due to substrates in the solution, which act as noise sources for baseline currents, and this noise has not been adequately addressed in previous technologies.
Innovation Solution
The use of electrolyte solutions containing ammonium ions and sulfate ions in the nanopore forming and measurement solutions to suppress noise from substrates and reduce baseline current noise.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If substrate is added to the solution to enable biomolecule transport, then biomolecule analysis can be performed, but baseline current noise increases
Solution Approach 1:
The patent introduces a specific electrolyte composition (ammonium formate, ammonium acetate, and formic acid) as an intermediary medium between the substrate and the measurement system. This intermediary solution suppresses the direct interaction between substrate and baseline current, thereby reducing noise while maintaining biomolecule transport capability
Solution Approach 2:
The patent changes the chemical parameters of the solution by using specific electrolytes (ammonium formate, ammonium acetate, formic acid) at controlled concentrations. This parameter change modifies the electrical properties of the solution to reduce substrate-induced noise while preserving the functional properties needed for biomolecule analysis
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach effectively reduces baseline current noise and allows for accurate measurement of blockade currents, enhancing the precision of biomolecule sequencing.
Implementation Method 1
a wall surface of the nanopore has an ion adsorption prevention structure that prevents the desorption and adsorption of ions contained in a solution filled in the first tank and/or the second tank
Implementation Method 2
a potential gradient is formed in the nanopore in accordance with the applied voltage. When a biomolecule such as DNA is introduced into the first liquid tank, the biomolecule is transported to the second liquid tank via the nanopore in response to the diffusion and the potential gradient
Implementation Method 3
the biomolecule is transported to the second liquid tank via the nanopore in response to the diffusion and the potential gradient
Implementation Method 4
forming a nanopore in the thin film by applying a first voltage between the first electrode and the second electrode
Data Source
AI summary
A biomolecule analysis method of the present disclosure includes: preparing a biomolecule analysis device including a thin film, a first liquid tank and a second liquid tank separated by the thin film, a first electrode disposed in the first liquid tank, and a second electrode disposed in the second liquid tank; and forming a nanopore in the thin film by applying a first voltage between the first electrode and the second electrode in a state where a nanopore forming solution is enclosed in the first liquid tank and the second liquid tank, wherein the nanopore forming solution contains ammonium ions and sulfate ions.