Uncultured Amniotic Cell Separation via Filtration
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Solution Overview
Problem
Current methods for preparing amniotic cell products from amniotic fluid are costly, time-consuming, and often result in lower concentrations of amniotic cell populations due to contamination with blood cells and other components, limiting their therapeutic effectiveness for regenerative medicine and wound healing.
Innovation Solution
A method involving the use of cell-separation filters to isolate uncultured amniotic cells and protein fractions from amniotic fluid, removing contaminants like blood cells, urea, and electrolytes, thereby producing products that are substantially free of these components, which can be used for tissue regeneration and wound healing.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If centrifugation methods are used to prepare uncultured amniotic cell products, then cell separation is achieved, but the product contains blood cells, cellular debris, and other contaminants that reduce therapeutic effectiveness
Solution Approach 1:
The patent applies the extraction principle by removing harmful components (blood cells, cellular debris, urea, electrolytes) from the amniotic fluid through a multi-step process involving filtration and centrifugation. The harmful substances are separated and discarded, leaving a purified amniotic cell product that maintains therapeutic effectiveness without contamination.
Solution Approach 2:
The patent segments the amniotic fluid into distinct components through sequential processing steps. Different cell types and molecular components are separated based on their physical and chemical properties, allowing selective isolation of amniotic cells while removing contaminants like blood cells and cellular debris.
2Quantity of substance
If cultured amniotic cell products are prepared through expansion in culture, then cell population increases, but the process is costly, time-consuming, and results in lower concentrations of some amniotic cell populations
Solution Approach 1:
The patent applies preliminary action by collecting and processing amniotic fluid immediately after amniocentesis or C-section, preserving cells in their natural state before culture. This eliminates the need for time-consuming expansion cultures while maintaining high cell population concentrations and diversity, as the cells are processed fresh rather than grown in culture over time.
Solution Approach 2:
The patent uses fresh amniotic cells directly from the fluid as a natural copy of the therapeutic product, eliminating the need for artificial culture expansion. The cells retain their native characteristics and concentrations without requiring prolonged cultivation periods.
3Quantity of substance
If cultured amniotic cell products are prepared through expansion in culture, then cell numbers increase, but the process is costly
Solution Approach 1:
The patent employs a simplified, cost-effective processing protocol that uses basic filtration and centrifugation equipment rather than expensive culture systems. The method processes amniotic fluid quickly and directly, avoiding costly culture media, incubators, and extended processing time, thereby reducing manufacturing costs while maintaining high cell population concentrations.
4Quantity of substance
If amniotic cells are cultured, then some populations divide more slowly than others, but this leads to lower concentrations of slower-dividing cell populations in the final product
Solution Approach 1:
The patent extracts all amniotic cell populations directly from the fresh amniotic fluid before culture, preserving the natural diversity and relative concentrations of different cell types. By processing cells immediately rather than allowing differential proliferation during culture, the method maintains the original stable composition of the cell population.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method provides a cost-effective and efficient way to produce amniotic cell products with higher concentrations of stem cells and proteins, suitable for therapeutic applications, including topical administration for skin, eye, joint, and spine treatments, enhancing tissue regeneration and healing.
Implementation Method 1
A method involving the use of cell-separation filters to isolate uncultured amniotic cells and protein fractions from amniotic fluid
Data Source
AI summary
Uncultured amniotic cell and protein fraction products derived from amniotic fluid and methods of preparing and using those compositions are provided. According to the methods of the present invention, uncultured amniotic cell and protein products may be derived from a large sample of amniotic fluid to provide a higher concentration of tissue regeneration components. Described are methods for separating uncultured amniotic cells or protein fractions from other components of amniotic fluid and the resulting uncultured amniotic cell and protein products. Furthermore, the present invention includes methods for delivering the uncultured amniotic cell and protein products to the skin and eye, including before, during, or after a treatment procedure.