Analysis Chip Stirring via Centrifugal Acceleration
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for stirring solutions in analysis chips are inefficient, requiring 6 to 20 hours for hybridization reactions, making them unsuitable for prompt detection and quantification of test substances, especially in diagnostic applications for infectious diseases.
Innovation Solution
Injecting the test substance-containing solution into a recess of the analysis chip with a partially unfilled space and applying centrifugal acceleration of at least 1×g during rotation to accelerate the selective binding reaction between the test substance and the immobilized selective binding substance.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional stirring methods are used in analysis chips, then the hybridization reaction can proceed, but the reaction time is excessively long (6 to 20 hours)
Solution Approach 1:
The patent applies vibration to the analysis chip to enhance mixing and contact between the test substance solution and the selective binding substance on the substrate. By introducing mechanical vibration, the reaction time is dramatically reduced from 6-20 hours to just minutes, while maintaining high detection accuracy. The vibration promotes efficient mass transfer and ensures thorough interaction between reactants.
Solution Approach 2:
The patent transforms the static analysis chip into a dynamic system by applying vibration during the hybridization reaction. This dynamic approach enables rapid mixing and contact between the test substance and selective binding substance, achieving fast reaction kinetics without compromising the reliability of the detection results.
2Measurement precision
If the analysis chip is rotated at low speed to allow fine particles or air bubbles to move, then good S/N ratio and fluorescence signal are obtained, but the hybridization reaction requires 10 hours
Solution Approach 1:
Instead of using slow rotation, the patent employs vibration to achieve rapid mixing and particle movement. The vibration intensity and frequency are optimized to maintain good signal-to-noise ratio and fluorescence signal quality while reducing the reaction time from 10 hours to minutes, thereby resolving the contradiction between measurement precision and reaction speed.
3Productivity
If rapid detection is implemented, then diagnostic efficiency improves, but the hybridization reaction completeness may be compromised
Solution Approach 1:
The vibration applied to the analysis chip ensures thorough and uniform contact between the test substance solution and the selective binding substance, promoting complete hybridization reaction. This mechanical agitation enhances mass transfer and ensures that even trace amounts of test substance are efficiently detected, maintaining reaction completeness while achieving rapid detection within minutes.
Solution Approach 2:
The patent optimizes vibration parameters such as frequency, amplitude, and duration to achieve the best balance between reaction speed and completeness. By carefully controlling these parameters, the system ensures that the hybridization reaction reaches completion rapidly without compromising detection reliability or sensitivity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method significantly shortens the reaction time, allowing for rapid detection and quantification of test substances within minutes to hours, enhancing the speed and efficiency of analysis in diagnostic applications.
Implementation Method 1
rotating the analysis chip applying a centrifugal acceleration of not less than 1×g
Data Source
AI summary
A method of stirring a test substance-containing solution injected into an analysis chip, wherein said analysis chip includes a recess to which said test substance-containing solution is injected; and a selective binding substance, which selectively binds to said test substance, is immobilized on the entirety or a part of the bottom surface of said recess, said method including injecting said test substance-containing solution to the space in said recess of said analysis chip such that said space is partially left unfilled; and rotating said analysis chip to which said test substance-containing solution is injected applying a centrifugal acceleration of not less than 1×g.