Analyte Detection via Brownian Motion Analysis
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Solution Overview
Problem
Existing analyte detection techniques require complex processes like B/F separation and suffer from non-specific adsorption, which interferes with specific analyte detection.
Innovation Solution
An analyte detection method that forms a complex of the analyte, a binding particle, and a trapping substance on a substrate, using Brownian motion indices like mean square displacement, diffusion coefficient, and average velocity to differentiate between specifically bound, non-specifically bound, and free particles without B/F separation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If B/F separation is performed to detect analyte, then detection specificity is improved, but process complexity increases
Solution Approach 1:
The invention extracts and eliminates the harmful non-specific adsorption component from the detection system. By using a dual-probe approach where one probe specifically binds to the analyte and another serves as a reference for non-specific binding, the method separates the specific signal from the non-specific background, achieving high detection specificity without complex separation procedures
Solution Approach 2:
The invention introduces a reference probe as an intermediary element that mediates between the non-specific adsorption and the detection system. This reference probe binds to the same surface as the analyte-specific probe but does not bind to the analyte, allowing the system to measure and subtract non-specific binding signals, thereby simplifying the overall detection process while maintaining high specificity
2Object-generated harmful factors
If B/F separation is performed to detect analyte, then non-specific adsorption is reduced, but operation time increases
Solution Approach 1:
The invention enables continuous measurement of both specific and non-specific binding signals simultaneously without interruption for separation steps. By implementing real-time dual-probe detection, the system continuously monitors analyte-specific binding while concurrently measuring non-specific adsorption, eliminating time losses associated with sequential separation operations
Solution Approach 2:
The invention replaces mechanical separation operations with an optical/detector-based measurement system. Instead of using physical methods to separate bound from free particles, the system uses detector units to optically detect and distinguish between specific and non-specific binding events, significantly reducing operation time while effectively managing non-specific adsorption
3Ease of operation
If conventional detection method is used, then detection process is simplified, but non-specific signal interference increases
Solution Approach 1:
The invention segments the detection signal into two distinct components: specific binding signal and non-specific adsorption signal. By using separate probe units that can be independently detected, the system divides the total signal into measurable segments, allowing the specific analyte signal to be isolated from the background non-specific signal while maintaining operational simplicity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method simplifies analyte detection by eliminating the need for B/F separation and reduces non-specific signals, allowing for accurate identification and quantification of analytes based on Brownian motion patterns.
Implementation Method 1
a detection step of determining a particle non-specifically bound on the substrate; an analyte-bound particle on the substrate; and a particle in a free state without binding to the trapping substance based on Brownian motion of the particle
Data Source
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AI summary
Disclosed is an analyte detection method, including detecting an analyte in such a state that a complex composed of the analyte in a sample of interest, a particle capable of binding to the analyte and a trapping substance capable of binding to the analyte is formed on a substrate, wherein the analyte is detected on the basis of an index associated with a behavior of the particle.