Analyte Detection via Singlet Oxygen Cleavage and Chemical Conversion
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Solution Overview
Problem
Current methods for detecting and quantifying analytes in biological samples, especially in fixed or preserved samples, are inadequate for accurate and efficient analysis, particularly for proteins or nucleic acids, which are crucial for cancer diagnosis and prognosis.
Innovation Solution
A method involving a binding compound linked to a releasable tag through a linker with a singlet oxygen-reactive group, where the tag is released upon binding to the analyte, and subsequently converted using a converting agent to produce a detectable product, allowing for enhanced detection and quantification via electrophoresis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional detection methods are used on fixed or preserved biological samples, then sample preservation is maintained, but detection precision and quantification accuracy deteriorate
Solution Approach 1:
The method performs preliminary actions by incorporating a converting agent into the detection system before actual measurement. The converting agent (such as a reducing agent like sodium borohydride or an oxidizing agent) is pre-positioned to convert intermediate products into detectable forms, ensuring that when fixed samples are analyzed, the detection precision is enhanced without requiring changes to the sample's preserved composition.
Solution Approach 2:
The invention introduces a converting agent as an intermediary substance that mediates between the analyte-binding step and the detection step. This intermediary converts the binding event into a chemically distinct intermediate product that can be reliably detected even in fixed samples, thereby improving measurement precision while maintaining compatibility with preserved sample composition.
2Productivity
If conventional detection methods are used, then procedural simplicity is maintained, but productivity and efficiency deteriorate
Solution Approach 1:
The detection method is segmented into distinct functional modules: (1) analyte binding to a binding compound, (2) conversion of the bound analyte to an intermediate product using a converting agent, and (3) detection of the intermediate product. This segmentation allows each step to be optimized independently and facilitates automation, thereby improving productivity without overwhelming complexity.
Solution Approach 2:
The invention utilizes parameter changes in the chemical state of the analyte during detection. By changing the chemical form of the bound analyte into a detectable intermediate product through the converting agent, the method achieves higher sensitivity and efficiency. This parameter change approach enables more accurate and efficient analysis while maintaining manageable procedural complexity through well-defined chemical transformations.
3Measurement precision
If conventional detection methods are used, then method simplicity is maintained, but detection sensitivity deteriorates
Solution Approach 1:
The invention replaces direct physical or mechanical detection methods with a chemical conversion system. Instead of directly detecting the bound analyte, the converting agent chemically transforms it into an intermediate product with enhanced detectability. This substitution of chemical mechanism for direct detection significantly improves sensitivity while the well-defined chemical reactions keep the method complexity manageable.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method enables precise and efficient detection and quantification of analytes in biological samples, including those preserved or fixed, improving the analysis of proteins and nucleic acids for diagnostic and prognostic purposes.
Implementation Method 1
releasing the releasable tag from the binding compound via a singlet oxygen mediated cleavage
Implementation Method 2
reacting the intermediate product with at least one converting agent thereby producing at least one converted product
Implementation Method 3
detection and/or quantification of the converted product occurs by electrophoresis
Data Source
AI summary
The invention relates to an enhanced method of detecting and/or quantifying at least one analyte in a sample.