Analytical Device Inner Reaction Chamber Trapping Element
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Solution Overview
Problem
Current analytical devices face challenges in efficiently processing large volumes of samples while maintaining high analyte recovery, due to complexity and cost, especially when dealing with dilute analytes or pathogens like E. coli, which can lead to reduced detection limits and increased fabrication costs.
Innovation Solution
An integrated analytical device with a reaction component featuring an inner chamber and a capping component, including a trapping element and reagent receiving compartment, allows for optical interrogation and efficient processing of samples, using a membrane or matrix to trap analytes and facilitate reagent addition, enabling high analyte recovery and detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If complex automation procedures are used to isolate analytes, then analytical capability is improved, but analyte loss and device complexity increase
Solution Approach 1:
The patent combines multiple analytical functions (sample introduction, analyte trapping, reagent addition, and detection) into a single integrated microchamber system. The microchamber serves as both the reaction vessel and the trapping medium container, eliminating the need for separate isolation and analysis equipment, thus reducing device complexity while maintaining analytical capability.
Solution Approach 2:
The trapping medium performs multiple functions simultaneously: it traps analytes from the sample, serves as the reaction environment for reagent addition, and acts as the medium for detection. This multi-functionality eliminates the need for separate steps and equipment for each operation, reducing overall device complexity.
2Measurement precision
If complex automation procedures are used to isolate analytes, then analytical capability is improved, but analyte recovery decreases
Solution Approach 1:
By combining the trapping step and analysis step into a single integrated microchamber system, the patent eliminates multiple transfer operations between different equipment. The analyte is trapped directly in the microchamber and remains there for subsequent reagent addition and detection, preventing loss that would occur during transfers between separate devices.
Solution Approach 2:
The trapping medium is pre-loaded into the microchamber before sample introduction. This preliminary preparation ensures that the trapping mechanism is already in place when the sample arrives, allowing immediate capture of analytes without requiring complex post-sampling isolation procedures that could lead to analyte loss.
3Ease of manufacture
If integrated analytical device is simplified for ease of manufacture, then fabrication cost decreases, but sample processing capability may be limited
Solution Approach 1:
The device is segmented into distinct functional components (input port, microchamber with trapping medium, reagent addition port, output port) that can be manufactured separately using simple techniques and then assembled. This segmentation allows each component to be optimized for easy manufacture while the integrated system maintains full sample processing capability.
Solution Approach 2:
The trapping medium utilizes porous materials that can be easily loaded into the microchamber and provide high surface area for analyte capture. These porous materials are commercially available and can be incorporated into simple device structures, maintaining both ease of manufacture and effective sample processing capability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device effectively captures and quantifies analytes with high detection efficacy, as demonstrated by the ability to detect 80% or more of E. coli cells, and maintains a high retention rate with minimal sample loss, while being cost-effective and simple to operate.
Implementation Method 1
a trapping element disposed therein for interaction with the one or more analyte
Implementation Method 2
a trapping element disposed therein for interaction with the one or more analyte
Data Source
AI summary
The present technology relates to an analytical device for quantitative or qualitative analysis of one or more analytes in a sample. The analytical device comprises a reaction component and a capping component. The reaction component is capable of performing an analysis on a sample placed within an inner reaction chamber that is connected to an output end and conduit that eliminates the air or liquid component of the sample. Once the output end is sealed, the inner reaction chamber may be used as an extraction, reaction and analysis chamber with an optically clear path for interrogation of the sample. The analytical device may also feature a sealing device that seals the inner reaction chamber during analysis. The sealing device may also have an inner chamber for accommodating lyophilized reagents that can be added at any point during the procedure.