1,5-Anhydroglucitol Analysis Kit with Integrated Chromogenic Reagent
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Solution Overview
Problem
Conventional 1,5-anhydroglucitol (1,5-AG) quantitative analysis methods are limited by the separate configuration of chromogenic reagents, preventing miniaturization and suffer from poor coloring ability under low-concentration hydrogen peroxide conditions, necessitating the development of a miniaturized kit with enhanced analytical capabilities.
Innovation Solution
A kit comprising a glucose removal reagent, a glucitol reaction reagent, and a chromogenic agent, with the latter being DA-67, fixed in solid state, along with stabilizers like sucrose, to enhance analytical capability and maintain enzyme activity over extended periods.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Volume of moving object
If conventional chromogenic reagents (4-AAP with HTIB or TOOS) are used for 1,5-AG quantitative analysis, then the analysis can be performed, but the kit cannot be miniaturized and requires large biochemical equipment
Solution Approach 1:
The patent combines multiple reagent functions into a single integrated chromogenic reagent composition. Instead of using separate reagents (4-AAP with HTIB or TOOS), the invention merges these into one composition that contains all necessary components for the chromogenic reaction, enabling kit miniaturization while maintaining analytical functionality
Solution Approach 2:
The chromogenic reagent composition is designed to perform multiple functions: it serves as both the chromogenic agent and the enzyme reaction substrate, and can detect both glucose and 1,5-AG. This multi-functionality reduces the number of separate reagents needed, facilitating kit miniaturization
2Measurement precision
If conventional chromogenic reagents are used, then the analysis can be performed, but the coloring ability is poor under low-concentration hydrogen peroxide conditions
Solution Approach 1:
The patent modifies the chemical parameters of the chromogenic reagent by selecting specific compounds (HTIB or TOOS) with enhanced sensitivity to low concentrations of hydrogen peroxide. The reagent composition is optimized to produce sufficient color change even when H2O2 concentration is low, thereby improving measurement precision under low-substance conditions
Solution Approach 2:
The chromogenic reagent is designed with specific local properties: HTIB or TOOS is selected as the chromogenic agent because these compounds have enhanced reactivity and coloring ability at low H2O2 concentrations. The reagent composition is locally optimized for maximum sensitivity in the detection zone
3Ease of operation
If the glucitol reaction reagent is stored in liquid state, then it is easy to use, but the enzyme activity decreases rapidly over time
Solution Approach 1:
The patent utilizes phase transition by storing the glucitol reaction reagent in solid state (lyophilized or dried form) rather than liquid state. This phase change preserves enzyme activity over extended periods (326 days), while the reagent can be reconstituted to liquid form immediately before use, maintaining ease of operation
Solution Approach 2:
The reagent is prepared in advance in a stable solid state, preserving enzyme activity. The reconstitution to liquid form is performed preliminarily before the actual measurement, ensuring both long-term stability and ease of use when needed
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The kit enables precise quantification of 1,5-AG with improved miniaturization and stability, allowing for accurate analysis even under low-concentration conditions, maintaining enzyme activity for up to 326 days.
Implementation Method 1
a first composition comprising a glucose removal reagent for removing at least a portion of glucose in a sample
Implementation Method 2
a second composition comprising a glucitol reaction reagent for enzyme reaction of 1,5-anhydroglucitol included in the sample from which at least a portion of glucose has been removed
Implementation Method 3
a third composition comprising a chromogenic agent being oxidized from hydrogen peroxide (H2O2) generated by enzyme reaction of the 1,5-anhydroglucitol
Data Source
AI summary
The kit for quantitative analysis of 1,5-anhydroglucitol (1,5-AG) according to an embodiment of the present application may comprise: a first composition comprising a glucose removal reagent for removing at least a portion of glucose in a sample; a second composition comprising a glucitol reaction reagent for enzyme reaction of 1,5-anhydroglucitol included in the sample from which at least a portion of glucose has been removed; and a third composition comprising a chromogenic agent being oxidized from hydrogen peroxide (H2O2) generated by enzyme reaction of the 1,5-anhydroglucitol.