1,5-Anhydroglucitol Kit with Solid-State DA-67 Chromogenic Reagent
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Solution Overview
Problem
Conventional 1,5-anhydroglucitol (1,5-AG) quantitative analysis methods are limited by the separate configuration of chromogenic reagents, preventing miniaturization and having poor analytical capabilities under low-concentration hydrogen peroxide conditions.
Innovation Solution
A kit and method for 1,5-AG analysis comprising a glucose removal reagent, a glucitol reaction reagent, and a chromogenic agent, with the latter being DA-67, are fixed in solid state to enhance analytical capability and enable miniaturization.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Volume of moving object
If conventional chromogenic reagents (4-AAP with HTIB or TOOS) are used for 1,5-AG quantitative analysis, then the analysis can be performed with established reagents, but the kit cannot be miniaturized and device size remains large
Solution Approach 1:
The patent combines multiple reagent functions into a single integrated chromogenic reagent formulation. Instead of using separate 4-AAP and HTIB/TOOS reagents as in conventional methods, the invention merges these components into one reagent system that performs both the coupling reaction and chromogenic function, enabling kit miniaturization while maintaining analytical capability
Solution Approach 2:
The chromogenic reagent in this invention serves multiple functions simultaneously: it acts as both the coupling agent and the chromogenic substrate for hydrogen peroxide detection. This multi-functional design eliminates the need for separate reagent configurations and enables the kit to be miniaturized without sacrificing analytical performance
2Measurement precision
If conventional chromogenic reagents are used, then the analysis system can operate, but the coloring ability under low-concentration hydrogen peroxide conditions is insufficient
Solution Approach 1:
The invention modifies the chromogenic reagent parameters to enhance its sensitivity to low concentrations of hydrogen peroxide. By adjusting the chemical structure and composition of the chromogenic agent, the reagent system achieves superior coloring ability even when hydrogen peroxide is present at low concentrations, thereby improving detection sensitivity for 1,5-AG quantification
3Volume of moving object
If chromogenic reagents are fixed in solid state to enable miniaturization, then the kit can be miniaturized, but the reagents may lose stability and analytical precision
Solution Approach 1:
The patent optimizes the physical and chemical parameters of the chromogenic reagent formulation to maintain stability in solid state. By carefully adjusting reagent concentrations, pH buffers, and stabilizing agents, the invention ensures that the fixed reagents retain their analytical precision and reliability while enabling kit miniaturization
Solution Approach 2:
The invention uses composite reagent formulations that combine multiple components in specific ratios to enhance stability in solid state. The chromogenic reagent system includes stabilizing agents and protective compounds that prevent degradation during storage, maintaining analytical precision even when reagents are fixed in solid form for miniaturized kit configuration
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The kit maintains high analytical precision and enables miniaturization, with the DA-67 chromogenic agent showing superior performance in differentiating hydrogen peroxide in low concentrations and stabilizing glucitol reaction reagents for extended storage periods.
Implementation Method 1
a third composition comprising a chromogenic agent which is oxidized from hydrogen peroxide (H2O2) generated by enzyme reaction of the 1,5-anhydroglucitol
Implementation Method 2
a second composition comprising a glucitol reaction reagent for enzyme reaction of 1,5-anhydroglucitol included in the sample
Data Source
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AI summary
The kit for quantitative analysis of 1,5-anhydroglucitol (1,5-AG) according to an embodiment of the present application may comprise: a first composition comprising a glucose removal reagent for removing at least a portion of glucose in a sample; a second composition comprising a glucitol reaction reagent for enzyme reaction of 1,5-anhydroglucitol included in the sample from which at least a portion of glucose has been removed; and a third composition comprising a chromogenic agent being oxidized from hydrogen peroxide (H2O2) generated by enzyme reaction of the 1,5-anhydroglucitol.