Anionic Manganese Oxide Nanoparticles for Low-Toxicity Nucleic Acid Scavenging
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Solution Overview
Problem
Existing cationic nucleic acid-binding polymers and nanoparticles pose cytotoxicity risks and are not effective in safely scavenging cell-free nucleic acids to alleviate inflammation and limit cancer metastasis, while anionic nanoparticles with negative surface charges offer a safer alternative.
Innovation Solution
Development of biodegradable anionic manganese oxide nanoparticles that scavenge cell-free nucleic acids, inhibiting Toll-like receptor activation and reducing inflammation and cancer metastasis by binding and neutralizing inflammatory molecules.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If cationic nucleic acid-binding polymers and nanoparticles are used to scavenge cell-free nucleic acids, then inflammation and cancer metastasis are reduced, but cytotoxicity increases
Solution Approach 1:
The patent inverts the conventional approach by using anionic (negatively charged) nanoparticles instead of cationic (positively charged) nanoparticles. This reversal of charge polarity resolves the cytotoxicity issue while maintaining the ability to scavenge cell-free nucleic acids through electrostatic interactions with positively charged DNA regions
Solution Approach 2:
The patent changes the surface charge parameter of the nanoparticles from positive to negative. This parameter change fundamentally alters the interaction profile with biological systems, reducing cytotoxicity while preserving nucleic acid scavenging capability through alternative electrostatic mechanisms
2Object-affected harmful factors
If anionic nanoparticles with negative surface charges are used, then cytotoxicity is reduced, but nucleic acid scavenging efficiency may be compromised
Solution Approach 1:
The patent applies local quality by creating heterogeneous surfaces on the anionic nanoparticles that contain both negatively charged regions (for low cytotoxicity) and positively charged patches (for nucleic acid binding). This local variation in surface charge distribution allows simultaneous achievement of low toxicity and high scavenging efficiency
Solution Approach 2:
The patent employs composite material strategies by combining materials with different surface charge characteristics or by functionalizing anionic nanoparticle surfaces with cationic moieties. This creates a composite structure that exhibits both low cytotoxicity and high nucleic acid scavenging capability
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The anionic manganese oxide nanoparticles effectively scavenge cell-free nucleic acids, reducing inflammation and metastasis with low cytotoxicity, and inhibit Toll-like receptor activation, providing a safer and more effective treatment for inflammatory and cancerous conditions.
Implementation Method 1
anionic manganese oxide nanoparticles that scavenge cell-free nucleic acids... binding and neutralizing inflammatory molecules
Data Source
AI summary
The anionic manganese oxide nanoparticle nucleic acid scavengers are biodegradable anionic scavengers with low cytotoxicity, which are able to scavenge (bind) cell-free nucleic acids (e.g., extracellular ssRNA, dsRNA, and unmethylated DNA), providing treatment for various medical conditions. The main component of the scavenger is manganese oxide, which may be synthesized by using a manganese compound (e.g., manganese acetate) and an acid (e.g., tannic acid) at high temperature (e.g., 100-150° C.). Synthesis may be performed by mixing a manganese compound and an acid in water forming a mixture, which is stirred, heated, and allowed to cool. The anionic manganese oxide nanoparticles are extracted from the cooled mixture. The typical size of the resultant nanomaterials ranges from 30 to 100 nm; the zeta potential of the as-prepared nanomaterials is about −20 mV. The nanoparticles have various uses, including administration to a subject to treat inflammation or to treat cancer.