Anionic Citrus Junos Peptide Composition for Fine Dust Protection
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing cosmetic compositions fail to effectively prevent the attachment of fine dust and air pollution and alleviate skin irritation and wrinkles due to insufficient utilization of anionic peptides derived from natural plant extracts.
Innovation Solution
A cosmetic composition is developed using low-molecular-weight anionic peptides isolated from Citrus junos seeds, prepared through a process involving enzymatic hydrolysis and cation exchange resin separation, to enhance skin absorption and electrostatic repulsion against fine dust.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If simple natural plant extracts are used as cosmetic ingredients, then the formulation is easy to produce, but the effectiveness in removing fine dust and addressing skin troubles is insufficient
Solution Approach 1:
The patent applies parameter changes by converting regular plant proteins into low-molecular-weight peptides through enzymatic hydrolysis, changing the molecular weight parameter to enhance skin penetration and effectiveness. The peptides are then converted into anionic forms by adjusting pH or adding anionic groups, further changing chemical parameters to achieve electrostatic repulsion against fine dust. This resolves the contradiction by maintaining natural plant origins (ease of manufacture) while achieving superior fine dust removal through parameter modification.
Solution Approach 2:
The patent creates composite functionality by combining multiple properties in the anionic peptide: low molecular weight for skin penetration, anionic charge for electrostatic repulsion of fine dust, and peptide structure for skin compatibility. This composite approach integrates multiple mechanisms (enzymatic hydrolysis, anion conversion, electrostatic interaction) into a single ingredient that simultaneously addresses ease of formulation and high effectiveness.
2Quantity of substance
If high-molecular-weight proteins are extracted from plant materials, then the material is readily available, but the skin absorption rate is low
Solution Approach 1:
The patent applies segmentation by breaking down large plant proteins into smaller peptide units through enzymatic hydrolysis using proteases. This segmentation reduces the molecular weight from high-molecular-weight proteins to low-molecular-weight peptides (typically below 10,000 Da), enabling better skin penetration while maintaining the beneficial amino acid sequences. The segmentation resolves the contradiction by preserving protein availability from plant sources while achieving rapid skin absorption through size reduction.
Solution Approach 2:
The patent changes the molecular weight parameter through controlled enzymatic hydrolysis, transforming high-molecular-weight proteins into low-molecular-weight peptides. This parameter change directly addresses the absorption rate issue while maintaining the natural plant origin. The hydrolysis process selectively cleaves peptide bonds to produce optimal molecular weight ranges that balance stability and penetration.
3Object-affected harmful factors
If regular peptides are used in cosmetic compositions, then they are biocompatible, but they cannot effectively prevent fine dust attachment due to lack of negative charge
Solution Approach 1:
The patent changes the electrical charge parameter of peptides by converting them into anionic forms. This is achieved through pH adjustment to above the isoelectric point or by incorporating anionic amino acids (aspartic acid, glutamic acid) or anionic groups (sulfonate, carboxylate). The anionic charge creates electrostatic repulsion against negatively charged fine dust particles, enabling effective prevention of dust attachment while maintaining peptide biocompatibility.
Solution Approach 2:
The patent converts the naturally negative charge of fine dust (which causes adhesion to skin) into a benefit by using anionic peptides that exploit electrostatic repulsion. Instead of trying to remove the negative charge from dust (which is harmful), the invention uses negative-charged peptides to repel the dust, turning the electrical property into a protective mechanism.
4Manufacturing precision
If complex purification processes are used to isolate functional peptides, then the purity is high, but the manufacturing complexity increases
Solution Approach 1:
The patent uses parameter changes to simplify purification: adjusting pH to exploit isoelectric point differences for precipitation, using solvent precipitation to separate peptides from proteins, and applying ultrafiltration with specific molecular weight cutoffs. These parameter-based separations achieve high peptide purity without requiring complex chromatographic procedures, resolving the contradiction between purity and manufacturing simplicity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The composition effectively prevents fine dust adsorption, alleviates skin irritation, and reduces wrinkles by inhibiting nitric oxide production and inflammatory cytokine expression, demonstrating improved skin barrier protection.
Implementation Method 1
inducing a primary enzymatic reaction using a subtilisin enzyme derived from Bacillus subtilis
Implementation Method 2
subtilisin enzyme derived from Bacillus subtilis
Implementation Method 3
inducing a secondary enzymatic reaction using pepsin
Implementation Method 4
pepsin
Implementation Method 5
separating a low-molecular-weight anionic peptide from the supernatant obtained in step (e) or a solution obtained by dissolving a powder obtained by lyophilizing the supernatant in distilled water using a cation exchange resin
Implementation Method 6
research is being conducted on substances that can prevent the adhesion of fine dust or can remove fine dust based on electrostatic repulsion
Data Source
AI summary
The present invention relates to a cosmetic composition containing a low-molecular weight anionic peptide isolated from Citrus junos seeds. More specifically, the present invention relates to a method for isolating a low-molecular weight peptide from Citrus junos seeds and processing same into a low-molecular weight anionic peptide, and a cosmetic composition including same as an active ingredient.