Annelid Hemoglobin Lyophilization Stabilizer

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Solution Overview

Problem

Current methods for storing and transporting hemoglobin oxygen carriers (HBOCs) require refrigeration, which is costly and logistically challenging, and lyophilization can degrade hemoglobin molecules, impairing their oxygen-binding functionality.

Innovation Solution

A process for lyophilizing extracellular hemoglobin from annelids using a stabilizer such as trehalose and ascorbic acid, which preserves the quaternary structure and functionality of the hemoglobin, allowing for stable and practical storage and transportation without refrigeration.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If hemoglobin is stored in solution form, then its oxygen-binding functionality is maintained, but refrigeration is required which increases storage cost and logistical complexity

Engineering Contradiction:

Improveoxygen-binding functionalityVSAvoidstorage and transportation logistics

Core Design Contradiction:

ReliabilityVSDevice complexity

Solution Approach 1:

The patent changes the physical state parameter of hemoglobin from liquid solution to solid lyophilized powder form. This parameter change eliminates the need for refrigeration while preserving oxygen-binding functionality through the protective action of stabilizers during the lyophilization process

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

Stabilizers (such as sugars, proteins, or other protective agents) are introduced as intermediary substances that mediate between the hemoglobin and the harsh lyophilization conditions. These stabilizers protect the hemoglobin structure during freezing and drying, enabling long-term storage without refrigeration while maintaining functionality

Inventive Principle:

Principle #24Intermediary (Mediator)

2Ease of operation

If hemoglobin is lyophilized to enable storage without refrigeration, then storage and transportation become easier, but the hemoglobin molecules are degraded and lose oxygen-binding functionality

Engineering Contradiction:

Improvestorage and transportationVSAvoidoxygen-binding functionality

Core Design Contradiction:

Ease of operationVSReliability

Solution Approach 1:

Stabilizers are added to the hemoglobin solution before lyophilization to provide protective cushioning against the damaging effects of freezing and drying. This prior protection prevents structural degradation and preserves oxygen-binding functionality throughout the lyophilization process and subsequent storage

Inventive Principle:

Principle #11Beforehand cushioning (Prior cushioning)

Solution Approach 2:

The patent creates a composite material system combining hemoglobin with stabilizing agents. This composite structure provides both the desired ease of storage and transportation through lyophilization and maintains functionality through the protective stabilizer components

Inventive Principle:

Principle #40Composite materials

3Duration of action of stationary object

If conventional lyophilization is applied to hemoglobin, then storage without refrigeration is achieved, but the quaternary structure and functionality are impaired

Engineering Contradiction:

Improvestorage durationVSAvoidquaternary structure integrity

Core Design Contradiction:

Duration of action of stationary objectVSManufacturing precision

Solution Approach 1:

The patent optimizes lyophilization parameters (freezing rate, drying temperature, vacuum level) to minimize structural damage while achieving complete water removal. These controlled parameter changes enable long-term storage without refrigeration while preserving quaternary structure integrity

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

Stabilizers act as intermediary protective agents during lyophilization, maintaining the quaternary structure of hemoglobin through molecular interactions that prevent denaturation. This intermediary protection enables extended storage duration while maintaining manufacturing precision of the protein structure

Inventive Principle:

Principle #24Intermediary (Mediator)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The lyophilized hemoglobin maintains its structural and functional integrity, enabling effective oxygen transport and storage in a compact, easily transportable form, with trehalose and ascorbic acid providing protection against degradation.

Implementation Method 1

A process for the lyophilization of at least one extracellular hemoglobin, globin protomer or globin of annelids

Methodology Applied

Scientific EffectLyophilization: Freeze Drying

Implementation Method 2

when it is mixed with a specific stabilizer, can be lyophilized, while at the same time preserving its quaternary structure, its functionality and its efficacy

Methodology Applied

Scientific EffectStabilization:

Data Source

PatentUS12016956B2Annelid haemoglobin lyophilisation process

Publication Date: 2024.06.25 HEMARINA

AI summary

The present invention relates to a lyophilizate comprising at least one globin, one globin protomer or one extracellular hemoglobin of annelids, and a stabilizer chosen from disaccharides, polyols and antioxidants. The present invention also relates to a composition comprising: a solution comprising at least one globin, one globin protomer or one extracellular hemoglobin of annelids, and a stabilizer chosen from disaccharides, polyols and antioxidants.Finally, the present invention relates to a process for preparing the lyophilizate.