Antibiotic Diagnostic Kit Simultaneous Multi-Class Detection
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Solution Overview
Problem
Current methods for detecting antibiotics in the agri-food sector are limited by the inability to simultaneously detect multiple classes of antibiotics in a single operation, leading to lengthy analysis times and increased costs due to the need for multiple tests, which hampers effective foodstuff management and control.
Innovation Solution
A diagnostic kit and method that uses a single reaction mixture with multiple biological recognition molecules capable of detecting various analytes, allowing for simultaneous detection and identification of different antibiotic classes without interference, utilizing a nitrocellulose membrane with distinct capture zones for each analyte, and protein-A labeled with colloidal gold as a generic marker.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If multiple separate tests are performed for different antibiotic classes, then detection accuracy and specificity are maintained, but analysis time increases and productivity decreases
Solution Approach 1:
The patent combines multiple separate antibody-based detection systems into a single reaction mixture containing different antibodies specific to different antibiotic classes (beta-lactams, tetracyclines, sulfonamides). This merging allows simultaneous detection of multiple antibiotic classes in one test, maintaining detection accuracy while reducing analysis time and increasing productivity.
Solution Approach 2:
The invention creates a universal detection system that can identify multiple types of antibiotics through a single test procedure. The reaction mixture contains multiple antibodies that can simultaneously recognize different antibiotic classes, making the test multi-functional and eliminating the need for separate confirmation tests for each antibiotic type.
2Productivity
If a single multi-analyte test is performed, then productivity increases and analysis time decreases, but device complexity and difficulty of detecting and measuring increase
Solution Approach 1:
The patent segments the detection system into distinct functional components: multiple antibodies specific to different antibiotic classes, a reaction mixture containing all antibodies, and a readout system. Each antibody targets a specific antibiotic class, and the segmented approach allows simultaneous detection while managing complexity through modular design.
Solution Approach 2:
The invention uses antibodies as intermediary molecules that mediate between the antibiotic analytes and the detection system. These biological recognition molecules serve as specific mediators that enable simultaneous detection of multiple antibiotic classes without direct interference, simplifying the overall detection mechanism.
3Loss of information
If multiple antibiotics are detected simultaneously, then the ability to identify antibiotic class improves, but false negative results may occur due to interference between recognition principles
Solution Approach 1:
The patent applies local quality by assigning specific antibodies to detect specific antibiotic classes within the same reaction mixture. Each antibody has localized specificity for its target antibiotic class, allowing simultaneous detection without cross-interference. This localized recognition ensures accurate class identification while maintaining high reliability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid, single-step detection of multiple antibiotic classes in less than 30 minutes, reducing analysis time and costs while maintaining high sensitivity and specificity, thereby enhancing the efficiency of foodstuff control and surveillance.
Implementation Method 1
a set of various biological recognition molecules, capable of specifically detecting several distinct analytes
Implementation Method 2
protein-A labeled with colloidal gold as a generic marker
Data Source
Figure 1~2
AI summary
The invention concerns a diagnostic kit for simultaneous assay of antibiotics of different classes, characterized in that it comprises: a single reaction mixture containing at least one first labelled receptor, specific of recognition of ß-lactams, a second labelled receptor, specifically and competitively identifying a tetracycline and a biotinylated nucleic acid fragment, an antisulfamide antibody and a labelled sulfamide analogue; and a recovery system in the form of a solid support comprising a nitrocellulose membrane whereon are fixed in three separate testing zones, respectively an antibiotic with ß-lactam nucleus, an avidin and an antibody capable of specifically identifying the antisulfamide antibody, so that the labelling intensity detected on the recovery system at the three testing zones results independently from a competitive recognition of each antibiotic by its labelled receptor.