Antibody-Oligonucleotide Conjugates for Multiplex Biomarker Detection
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Solution Overview
Problem
Current diagnostic tools are inadequate for simultaneously detecting multiple protein biomarkers, limiting their ability to diagnose diseases at earlier stages and track complex cellular interactions.
Innovation Solution
The development of biomolecule-oligonucleotide conjugates, such as antibody-oligonucleotide bioconjugates, that can be formed with greater than 80% efficiency, allowing for high-yield and high-purity preparation for use in diagnostic assays.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If current diagnostic tools are used to detect multiple protein biomarkers, then the number of assays that can be performed simultaneously is limited, but the ability to diagnose diseases at earlier stages and track complex cellular interactions is compromised
Solution Approach 1:
The patent divides the detection system into multiple independent conjugate components, each targeting a specific biomarker. This segmentation allows simultaneous detection of multiple proteins using parallel conjugate-antibody-oligonucleotide complexes, increasing assay capacity while maintaining diagnostic accuracy through targeted detection of multiple biomarkers concurrently
Solution Approach 2:
The patent creates a universal detection platform where a single conjugate system can detect multiple different biomarkers through the use of multiple conjugates with different specificities. This multi-functional approach enables one assay system to perform multiple diagnostic functions simultaneously, improving both productivity and reliability
2Productivity
If conventional bioconjugation methods are used, then the preparation process is complex and time-consuming, but the yield and purity of the conjugates are insufficient
Solution Approach 1:
The patent incorporates the oligonucleotide onto the antibody during the conjugation process itself, rather than requiring separate preliminary steps. This preliminary integration of the detection component into the conjugate formation enables high-yield, high-purity conjugates to be prepared in a single operation, dramatically reducing preparation time while improving productivity
Solution Approach 2:
The patent merges the conjugation process with the oligonucleotide attachment process into a single integrated reaction. By combining these operations, the system eliminates multiple preparation steps, achieves higher conjugate yield and purity, and reduces the overall time required for conjugate preparation
3Adaptability or versatility
If existing diagnostic assays are used, then only single protein biomarkers can be detected, but the comprehensive understanding of cellular interactions and disease mechanisms is limited
Solution Approach 1:
The patent transitions from single-biomarker detection to multi-biomarker detection by adding an additional dimension of detection capability. The conjugate-antibody-oligonucleotide system enables simultaneous detection of multiple biomarkers in a single assay, providing comprehensive information about cellular interactions and disease mechanisms that was previously unavailable
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
These conjugates enable the detection of multiple molecular targets in a sample, facilitating more comprehensive diagnostic assays and improving clinical decision-making.
Implementation Method 1
forming biomolecule-oligonucleotide conjugates at greater than 80% efficiency from at least one or more modified biomolecules and at least one or more modified oligonucleotides
Implementation Method 2
biomolecule-oligonucleotide conjugates, such as antibody-oligonucleotide bioconjugates, that can be formed with greater than 80% efficiency
Data Source
AI summary
The present disclosure is directed to methods and/or uses of oligonucleotide conjugates for assays and flow cytometry detections and related systems and/or kits. Certain methods are directed to a method for detecting one or more biological targets of a sample in a detection assay, comprising: providing a molecular probe, comprising a binding moiety and an oligonucleotide sequence, to a sample comprising one or more biological targets; binding the one or more biological targets with the binding moiety; providing a detectable component to the sample, wherein the detectable component comprises a signal generating moiety conjugated to an oligonucleotide sequence complementary to the oligonucleotide sequence of the molecular probe; hydridizing the oligonucleotide sequence of the target-bound molecular probe to the detectable component; and detecting a signal generated from the hydridized detectable component. Various other embodiments, applications etc. are disclosed herein.