Antibody-Producing Cell Sorting for Non-Competitive Binding
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Solution Overview
Problem
Existing methods for sorting B cells and other antibody-producing cells fail to efficiently enrich and isolate cells that express antibody molecules with unique and desirable features, such as non-competition or blocking capabilities with respect to binding partners.
Innovation Solution
Methods are developed to detect and differentiate antibody-producing cells that express antibody molecules non-competitively or competitively with binding partners by using target antigens conjugated with detectable labels and specific binders, allowing for the collection of cells expressing non-competitive or blocking antibodies.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional B cell sorting methods are used, then cells can be isolated, but cells expressing antibody molecules with unique features (non-competitive or blocking capabilities) cannot be efficiently enriched
Solution Approach 1:
The target antigen is pre-bound with a first binder (competitor antibody) before contacting with antibody-producing cells. This preliminary action blocks competitive antibodies from binding, allowing only non-competitive antibodies to bind to the antigen and be detected/enriched through flow cytometry or sorting
Solution Approach 2:
A first binder (competitor antibody) is introduced as an intermediary to mediate the detection process. This binder competes with antibody-producing cells for binding to the target antigen, enabling differentiation between competitive and non-competitive antibodies through detectable labels
2Measurement precision
If conventional sorting methods are used, then antibody-producing cells can be collected, but blocking antibodies cannot be differentiated from non-blocking antibodies
Solution Approach 1:
The target antigen is pre-bound with a first binder before cell contact, creating a blocked state that prevents competitive/blocking antibodies from binding. This preliminary blocking action enables clear differentiation between blocking and non-blocking antibodies based on their ability to bind under these conditions
Solution Approach 2:
The binding conditions are changed by introducing a competitor binder and using detectable labels, transforming the detection parameter from simple antigen-binding to competitive/non-competitive binding differentiation, enabling precise identification of blocking antibodies
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The methods enhance the enrichment and isolation of antibody-producing cells that are non-competitive or blocking, improving the efficiency and specificity of antibody production processes.
Implementation Method 1
contacting a population of antibody-producing cells with: (1) the target antigen; and (2) the first binder to the target antigen, wherein the first binder is conjugated with a detectable label; to permit binding of cells that express antibody molecules that are non-competitive with the first binder to bind to the target antigen
Data Source
AI summary
Disclosed herein are methods for detecting cells expressing antibody molecules on the cell surface that bind to a targeted antigen without competing with one or more binding partners of the target antigen. Also disclosed herein are methods for detecting and differentiating cells expressing antibody molecules on the cell surface to a target antigen that are non-competing with one or more binding partners, from cells expressing antibody molecules to a target antigen that block binding by a binding partner. The methods disclosed herein allow for enrichment and isolation of cells expressing antibody molecules on the cell surface to a target antigen that are non-competing with one or more binding partners, and cells expressing antibody molecules on the cell surface to a target antigen that block binding by a binding partner.