Antibody Scaffold Deep Pocket HCDR3 RNA-Protein Binding
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Solution Overview
Problem
Current technologies lack the ability to effectively identify and generate antibodies with new binding specificities for RNA-protein complexes, limiting their application in therapeutic and diagnostic fields, particularly in cancer treatment.
Innovation Solution
Development of an antibody scaffold with a deep pocket and specific HCDR3 structure, allowing for the generation of antibodies with altered binding specificities through mutation and screening, targeting RNA-protein complexes such as polyadenylate-binding protein 1 (PABP-1) for therapeutic and diagnostic applications.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If conventional antibody technologies are used, then existing binding specificities are maintained, but the ability to identify antibodies with new binding specificities for RNA-protein complexes is limited
Solution Approach 1:
The patent applies preliminary action by pre-designing an antibody scaffold structure with a deep pocket and specific HCDR3 configuration before the actual antibody identification process. This pre-engineered scaffold is then used as a template for generating antibodies with novel binding specificities, improving both adaptability and identification efficiency for RNA-protein complexes
2Adaptability or versatility
If antibody scaffold with deep pocket and specific HCDR3 structure is used, then antibodies with new binding specificities can be generated, but the structural complexity increases
Solution Approach 1:
The patent applies local quality by introducing specific structural features (deep pocket and disulfide bonds) only in the critical HCDR3 region of the antibody scaffold, rather than complicating the entire antibody structure. This localized structural modification enables novel binding specificities while minimizing overall structural complexity
Solution Approach 2:
The patent employs composite materials by combining the antibody scaffold with RNA-protein complex targets, creating a composite system where the scaffold's deep pocket structure selectively binds to specific epitopes on the RNA-protein complexes, enabling generation of antibodies with new binding specificities
Data Source
AI summary
Provided herein are antibodies comprising a scaffold region and methods of using such antibodies to generate antibodies with binding specificities that differ from a parent antibody that comprises the scaffold region.