Anti-freezing Agent for Biological Tissue Protection
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Solution Overview
Problem
During the freezing treatment of tissues for malignant bone sarcoma, surrounding innocent normal tissues such as cartilage, bone, and tendon are often damaged, as existing methods fail to effectively protect these tissues from the destructive effects of rapid freezing and slow thawing.
Innovation Solution
A method involving the preparation of an anti-freezing agent by mixing ethylene glycol and water with dimethyl sulfoxide, followed by the addition of sodium polyacrylate, creates a protective solution with specific weight ratios to safeguard biological tissues during freezing treatments, preventing damage to cartilage, tendon, and ligament tissues.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If rapid freezing treatment is applied to kill tumor cells, then tumor cells are completely killed, but surrounding innocent normal tissues including cartilage, bone and tendon are destroyed
Solution Approach 1:
The patent introduces a protective colloid (intermediary substance) that acts as a mediator between the freezing treatment and normal tissues. This protective colloid forms a protective barrier around normal tissues, allowing the freezing treatment to effectively kill tumor cells while preventing the direct destructive effect on normal cartilage, bone and tendon tissues.
Solution Approach 2:
The patent applies preliminary protective measures by coating normal tissues with a protective colloid before the freezing treatment. This preliminary action creates a protective layer that counteracts the harmful freezing effect, enabling the subsequent rapid freezing to successfully eliminate tumor cells without causing equivalent damage to normal tissues.
2Reliability
If liquid nitrogen is used for freezing treatment, then tumor cells are completely killed through crystallization of water molecules, but the process is complex and requires precise temperature control
Solution Approach 1:
The protective colloid serves as an intermediary that simplifies the freezing process by providing a protective barrier, thereby reducing the need for complex temperature control systems and equipment while maintaining effective tumor cell killing through the freezing treatment.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The anti-freezing agent effectively protects cartilage and tendon tissues from fragmentation and apoptosis, maintaining tissue integrity and reducing moisture loss, as demonstrated by reduced damage and improved structural preservation in experimental groups compared to control groups.
Implementation Method 1
reduce the temperature of the bone with tumor cells to a quite low temperature, so that water molecules inside and outside the bone cells undergo crystallization to form ice crystals, which can destroy the cell membrane and make the tumor cells die
Implementation Method 2
mixing ethylene glycol and water to form an aqueous ethylene glycol solution
Data Source
AI summary
Disclosed are an anti-freezing agent for protecting a biological tissue from being damaged during a freezing treatment and a preparation method therefor. The method for preparing the anti-freezing agent involves mixing ethylene glycol, water and dimethylsulfoxide homogeneously to form a matrix, and then slowly adding sodium polyacrylate. The prepared anti-freezing agent is coated onto biological tissue during a freezing treatment such that damage to the biological tissue is reduced.