Antigen-Binding Molecule Quantification via Acid-Surfactant Extraction
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Solution Overview
Problem
Conventional methods for quantifying antigen-binding molecules bound to cells are not precise, as they indirectly detect the antibodies, leaving room for improvement in quantitative accuracy.
Innovation Solution
A method involving the preparation of a biological sample from cells expressing a target antigen, followed by the addition of an extraction solution containing an organic acid and a nonionic surfactant, to obtain an analyte for direct quantification of the antigen-binding molecule.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional indirect detection methods are used to measure antibody bound to target tissue, then the measurement process is simpler, but the quantitative precision is insufficient
Solution Approach 1:
The patent applies extraction by taking out the antigen-binding molecule from the biological sample using an extraction solution. This allows direct measurement of the extracted antibody, improving quantitative precision while managing procedural complexity through systematic extraction steps.
Solution Approach 2:
The patent introduces an extraction solution as an intermediary substance that facilitates the separation and extraction of antigen-binding molecules from cells. This mediator enables direct quantification while managing the complexity of the overall measurement process.
2Measurement precision
If direct extraction and measurement of antigen-binding molecules is performed, then quantitative precision improves, but the procedure becomes more complex
Solution Approach 1:
The patent utilizes parameter changes in the extraction solution (pH 1-3, organic acid composition, nonionic surfactant concentration) to optimize extraction efficiency. These controlled parameter changes enable direct and accurate quantification while maintaining operational feasibility through standardized conditions.
Solution Approach 2:
The patent employs a composite extraction solution containing organic acid and nonionic surfactant. This composite formulation enhances extraction efficiency and direct measurement accuracy while managing procedural complexity through a well-defined multi-component system.
3Reliability
If extraction solution with pH 1-3 containing organic acid and nonionic surfactant is used, then extraction efficiency and quantification accuracy improve, but the protocol complexity increases
Solution Approach 1:
The patent specifies precise parameter ranges for the extraction solution (pH 1-3, particular organic acid types, nonionic surfactant concentrations) to optimize extraction reliability. These controlled parameter specifications enable accurate and sensitive quantification while maintaining protocol manageability through defined conditions.
Solution Approach 2:
The patent uses a composite extraction solution combining organic acid and nonionic surfactant in specific ratios. This composite approach improves extraction reliability and quantification accuracy while managing protocol complexity through a systematic multi-component formulation with defined roles for each ingredient.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method enables accurate and sensitive quantification of antigen-binding molecules bound to cells, improving the precision of antibody drug evaluation and allowing for the analysis of antibody accumulation in both target and non-target tissues.
Implementation Method 1
an extraction step of adding an extraction solution to the biological sample to obtain an analyte
Implementation Method 2
the extraction solution comprises an organic acid and a nonionic surfactant
Data Source
AI summary
Provided is a method of quantifying an antigen-binding molecule bound to cells, comprising: a preparation step of preparing a biological sample derived from the cells, wherein the biological sample is homogenized, the cells express a target antigen bound specifically to the antigen-binding molecule, and the cells have been exposed to the antigen-binding molecule; an extraction step of adding an extraction solution to the biological sample to obtain an analyte, wherein the extraction solution comprises an organic acid and a nonionic surfactant, and the extraction solution has a pH of 1 or more and 3 or less; and a quantification step of analyzing the analyte to quantify the antigen-binding molecule.