Antimicrobial Composition for Controlled Hydrogen Peroxide Release

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Solution Overview

Problem

Existing antimicrobial compositions, such as those based on honey, suffer from variability in efficacy due to differences in honey composition, potential allergens, and processing complexities, and lack stability and sustained antimicrobial activity over time.

Innovation Solution

A composition comprising a purified enzyme that converts a substrate to release hydrogen peroxide, combined with a substrate and a solute, optimized for low water activity and controlled hydrogen peroxide release, providing enhanced antimicrobial efficacy and stability.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If natural honey is used as the basis for antimicrobial composition, then antimicrobial activity is achieved, but composition variability and efficacy inconsistency occur

Engineering Contradiction:
Improveantimicrobial efficacy consistencyVSAvoidhoney composition variability
Core Design Contradiction:
ReliabilityVSStability of the object's composition

Solution Approach 1:

The patent extracts the essential antimicrobial mechanism from honey (glucose oxidase enzyme activity) and separates it from the variable natural honey matrix. By using purified glucose oxidase enzyme combined with controlled substrate (glucose) and solute (fructose) components, the invention eliminates composition variability while preserving the hydrogen peroxide generation mechanism that provides antimicrobial activity.

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The patent controls key parameters including water activity (aw ≤ 0.6), pH (3.0-7.0), and specific component concentrations (glucose oxidase 0.01-1.0%, glucose 1-30%, fructose 10-80%) to ensure consistent antimicrobial efficacy. These parameter controls transform the variable natural honey into a standardized composition with reliable performance.

Inventive Principle:
Principle #35Parameter changes

2Reliability

If honey is processed for pharmaceutical application, then suitability for medical use is improved, but production cost and complexity increase

Engineering Contradiction:
Improvepharmaceutical suitabilityVSAvoidprocessing complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent segments the honey processing into distinct functional components: purified glucose oxidase enzyme, substrate (glucose), and solute (fructose). Each component can be independently controlled and processed, simplifying the overall manufacturing process while ensuring pharmaceutical-grade quality and consistency.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent creates a simplified copy of honey's antimicrobial mechanism using purified components rather than processing whole honey. This synthetic approach replicates the essential function (hydrogen peroxide generation) without requiring complex processing of natural honey, reducing both cost and complexity.

Inventive Principle:
Principle #26Copying

3Stability of the object's composition

If honey is used without sufficient free water, then stability is improved, but enzyme activity and hydrogen peroxide production are reduced

Engineering Contradiction:
Improvecomposition stabilityVSAvoidhydrogen peroxide generation efficiency
Core Design Contradiction:
Stability of the object's compositionVSReliability

Solution Approach 1:

The patent optimizes water activity to a specific range (aw ≤ 0.6) that balances stability and enzyme activity. This parameter control allows the composition to remain stable during storage while maintaining sufficient enzyme functionality to generate hydrogen peroxide when needed, resolving the contradiction between stability and activity.

Inventive Principle:
Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The composition achieves sustained hydrogen peroxide release at specific concentrations for extended periods, overcoming the limitations of natural honey and ensuring consistent antimicrobial activity.

Implementation Method 1

an enzyme that is able to convert a substrate to release hydrogen peroxide

Methodology Applied
Scientific EffectEnzyme catalysis: Enzyme

Implementation Method 2

convert a substrate to release hydrogen peroxide

Methodology Applied
Scientific EffectHydrogen peroxide generation: Hydrogen Peroxide

Data Source

PatentEP3946481B1Antimicrobial compositions
Publication Date: 2026.03.04 MATOKE HLDG
  • EP3946481B1 patent drawingFigure 1~4
  • EP3946481B1 patent drawingFigure 5~6
  • EP3946481B1 patent drawingFigure 7~8

AI summary

Liquid compositions are described which have enzyme that is able to convert a substrate to release hydrogen peroxide; substrate for the enzyme; and polymer. The substrate is less than 10% by weight of the composition and the composition does not comprise sufficient free water to allow the enzyme to convert the substrate, or has a water activity of 0.7 or less.