Antimicrobial Aqueous Solution with Sucrose Monolaurate Solubilizer
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Solution Overview
Problem
Current antimicrobial solutions are ineffective against gram-negative bacteria, particularly Pseudomonas, due to their resistance to lipophilic antibiotics and the limitations of existing antimicrobial agents like ε-polylysine and monoglycerol monolaurate, which face challenges with solubility and synergistic effects at typical concentrations.
Innovation Solution
An antimicrobial aqueous solution combining ε-polylysine with monoglycerol monolaurate or diglycerol monolaurate at lower concentrations, along with sucrose monolaurate as a solubilizer, to enhance solubility and permeability, thereby achieving broad-spectrum antimicrobial activity against gram-negative bacteria.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If lipophilic antibiotics are used to treat gram-negative bacteria, then they can effectively kill gram-positive bacteria, but they cannot penetrate the outer membrane of gram-negative bacteria due to resistance
Solution Approach 1:
The invention combines ε-polylysine (a cationic peptide) with monoglycerol monolaurate or diglycerol monolaurate (amphipathic antimicrobial agents) to create a composite antimicrobial system. This composite approach allows the solution to overcome the outer membrane barrier of gram-negative bacteria through synergistic action, where ε-polylysine disrupts the membrane structure and enables penetration of the amphipathic agents, achieving effective killing of gram-negative bacteria including Pseudomonas species that are resistant to conventional lipophilic antibiotics
2Reliability
If ε-polylysine and monoglycerol monolaurate are used at typical concentrations, then they show some antimicrobial activity, but their solubility is limited and synergistic effects are not achieved
Solution Approach 1:
The invention optimizes the concentration parameters of the antimicrobial components, using ε-polylysine at 1-100 μg/ml and monoglycerol monolaurate/diglycerol monolaurate at 1-50 μg/ml. Additionally, sucrose monolaurate is added at 0.1-100 μg/ml as a solubilizer to enhance the solubility of the amphipathic antimicrobial agents in aqueous solution, thereby achieving both high antimicrobial activity and adequate solubility that were not simultaneously attainable at typical concentrations
3Reliability
If conventional antiseptic solutions are used to prevent epidemic diseases, then they can sterilize surfaces, but they have toxicities to humans and residual properties that prevent prophylactic dispersion
Solution Approach 1:
The invention uses naturally occurring substances (ε-polylysine from Streptomyces albulus, and glycerol monolaurate/diglycerol monolaurate) at optimized low concentrations (microgram per ml range), which significantly reduces human toxicity compared to conventional antiseptics. The addition of sucrose monolaurate further enhances safety by improving solubility and reducing irritation, enabling prophylactic use in schools, kindergartens, and public spaces where conventional antiseptics would be too toxic for continuous exposure
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution effectively restricts the growth of gram-negative bacteria, including Pseudomonas, by synergistically enhancing the antimicrobial activity of ε-polylysine and monolaurate, while being biodegradable and safe for use, with applications in various settings.
Implementation Method 1
add a sucrose monolaurate as a solubilizer into the solution
Implementation Method 2
synergistically enhancing the antimicrobial activity of ε-polylysine and monolaurate
Implementation Method 3
enhancing the antimicrobial activity of ε-polylysine and monolaurate, thereby achieving broad-spectrum antimicrobial activity against gram-negative bacteria
Data Source
AI summary
The antimicrobial aqueous solution of the present invention comprises an original undiluted aqueous solution containing: 25 μg/ml to 50 μg/ml of an amphipathic preservative comprising a monoglycerol monolaurate; and 12.5 μg/ml to 30 μg/ml of an ε-polylysine. In preparing the antimicrobial aqueous solution, dissolution of the amphipathic antimicrobial can be promoted by addition of a sucrose monolaurate and by heating to 35° C. to 60° C. This antimicrobial aqueous solution can be used effectively, even within a range of concentrations lower than concentrations to be typically used for amphipathic antimicrobials. It is possible to adopt a diglycerol monolaurate, instead of the monoglycerol monolaurate.