Apelin-Enriched Stem Cell Culture Medium for Proliferation

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Solution Overview

Problem

Stem cells experience reduced self-renewal ability and telomerase activity when repeatedly cultured or subjected to external stimuli, leading to senescence, and existing medium compositions are costly and inefficient, requiring frequent subculturing and reducing cell yield.

Innovation Solution

A medium composition containing apelin, which enhances the self-renewal ability of stem cells without altering their morphology or activity, allowing for prolonged culturing without senescence, and can be supplemented with antioxidants like CoQ10 and oltipraz to improve proliferation.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If stem cells are repeatedly cultured in conventional media, then cell yield increases, but self-renewal ability decreases and senescence occurs

Engineering Contradiction:

Improvecell yieldVSAvoidself-renewal ability

Core Design Contradiction:

ProductivityVSReliability

Solution Approach 1:

The patent changes the chemical composition parameters of the culture medium by incorporating apelin and antioxidants (CoQ10, oltipraz) to modify the cellular microenvironment. This parameter change maintains stem cell self-renewal ability while enabling prolonged culturing, thus resolving the contradiction between increasing cell yield and preserving self-renewal capacity

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

Apelin acts as an intermediary substance that mediates between the culture medium and stem cells, signaling to maintain self-renewal capacity. The antioxidants serve as intermediaries that protect against oxidative stress during repeated passaging, allowing high productivity without loss of cellular functionality

Inventive Principle:

Principle #24Intermediary (Mediator)

2Ease of manufacture

If conventional medium compositions are used, then culturing process is simple, but cell proliferation efficiency is low and subculturing frequency is high

Engineering Contradiction:

Improveculturing process simplicityVSAvoidcell proliferation efficiency

Core Design Contradiction:

Ease of manufactureVSProductivity

Solution Approach 1:

By modifying the medium composition parameters to include apelin and antioxidants, the patent achieves enhanced cell proliferation efficiency without complicating the overall culturing process. The added components work synergistically to reduce subculturing frequency while maintaining process simplicity

Inventive Principle:

Principle #35Parameter changes

3Productivity

If stem cells are cultured for long periods, then cell yield increases, but telomerase activity decreases and senescence occurs

Engineering Contradiction:

Improvecell yieldVSAvoidculturing duration

Core Design Contradiction:

ProductivityVSDuration of action of stationary object

Solution Approach 1:

Antioxidants (CoQ10 and oltipraz) act as intermediaries that protect telomerase activity from oxidative degradation during prolonged culturing. This mediation allows extended culture duration to achieve higher cell yields without the typical decline in telomerase activity and subsequent senescence

Inventive Principle:

Principle #24Intermediary (Mediator)

Data Source

PatentUS9982234B2Culture medium composition for improving regenerative capacity of stem cells, and stem cell culturing method using same

Publication Date: 2018.05.29 R BIO CO LTD

AI summary

The present invention relates to a medium composition for enhancing the self-renewal ability of stem cells, which contains apelin, and a method of culturing stem cells using the medium composition. According to the present invention, stem cells can be effectively cultured to proliferate without changing the characteristics of the cells, and the telomerase activity of stem cells can be increased. Thus, the efficacy of cell therapy based on stem cells can be significantly improved.